Quality evaluation of Mongolian medicine Sendeng-4 based on qualitative and quantitative analysis combined with chemical pattern recognition
- VernacularTitle:基于定性、定量及化学模式识别分析的蒙药森登-4质量评价
- Author:
Fengye ZHOU
1
;
Jun LI
2
;
Qian ZHANG
2
;
Rongjie LI
1
;
Wei ZHANG
2
;
Jing LIU
2
;
Fang WANG
2
;
Shengnan LI
3
Author Information
1. Dept. of Pharmacy，the Affiliated Hospital of Inner Mongolia Medical University，Hohhot 010030，China
2. College of Pharmacy，Inner Mongolia Medical University，Hohhot 010110，China;Inner Mongolia Autonomous Region Engineering Research Center of New Pharmaceutical Screening，Hohhot 010110，China
3. Dept. of Endocrinology，the Affiliated Hospital of Inner Mongolia Medical University，Hohhot 010030，China
- Publication Type:Journal Article
- Keywords:
Sendeng-4;
qualitative analysis;
quantitative analysis;
chemical pattern recognition analysis;
content
- From:
China Pharmacy
2025;36(9):1040-1045
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To evaluate the quality of Mongolian medicine Sendeng-4 based on qualitative and quantitative analysis combined with chemical pattern recognition， in order to provide the reference for its quality control. METHODS The chemical components in Sendeng-4 were analyzed qualitatively by HPLC-Q-Exactive-MS. The contents of 16 components （methyl gallate， ethyl gallate， epicatechin， dihydromyricetin， genipin-1-O-β-D-gentiobioside， caffeic acid， catechin， corilagin， deacetylasperulosidic acid methyl ester， rutin， geniposide， luteolin， myricetin， quercetin， ferulic acid， and toosendanin） in 15 batches of Sendeng-4 （sample S1-S15） were quantitatively analyzed by HPLC-MS/MS. Cluster analysis （CA）， principal component analysis （PCA）， and orthogonal partial least squares discriminant analysis were conducted and variable importance projection （VIP） value greater than 1 was used as the index to screen the differential components. RESULTS A total of 73 chemical components were identified in Sendeng-4， including 20 flavonoids， 16 tannins， 14 organic acids， etc. According to the quantitative analysis， the results exhibited that the average contentsthe of above 16 components in 15 batches of Sendeng-4 were 3.683-7.730， 2.391-6.952， 2 275.538-4 377.491， 2 699.188-3 537.924， 858.266-1 377.393， 3.366-11.003， 140.624-315.683，414.629-978.334， 285.501-1 510.457， 27.799-48.325， 3 625.415-6 309.563， 0.506-0.656， 442.337-649.283， 47.093-59.736， 12.942-15.822， 127.738-326.649 μg/g， respectively. According to the results of CA and PCA， 15 batches of samples could be clustered into two categories: S1-S3， S5-S6， S9-S10 and S13 were clustered into one category； S4， S7-S8， S11-S12， S14-S15 were clustered into one category. VIP values of geniposide， epicatechin， deacetylasperulosidic acid methyl ester and genipin-1-O- β-D-gentiobioside were all greater than 1. CONCLUSIONS HPLC-Q-Exactive-MS and HPLC-MS/MS techniques are employed for the qualitative and quantitative analysis of Sendeng-4. Through chemical pattern recognition analysis， four differential components are identified: geniposide， epicatechin， deacetylasperulosidic acid methyl ester， and genipin-1-O-β-D-gentiobioside.
