Effect of hypertension on cardiovascular fibrosis and sFRP2 expression in rats
10.12206/j.issn.2097-2024.202409055
- VernacularTitle:高血压对大鼠心血管纤维化及sFRP2表达的影响
- Author:
Yao XU
1
;
Chunhui MA
1
;
Zhiyong LI
1
Author Information
1. Department of Pathology, Faculty of Medical Imaging, Naval Medical University, Shanghai 200433, China.
- Publication Type:Originalarticles
- Keywords:
SHR rats;
hypertension;
collagen fibers;
elastic fibers;
sFRP2
- From:
Journal of Pharmaceutical Practice and Service
2025;43(4):180-184
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of spontaneous hypertension on the remodeling of cardiac and aortic tissues in rats, with special attention to the changes in the content of collagen fibers, elastic fibers and secreted Frizzled-related protein 2 （sFRP2） in cardiac and aortic tissues. Methods 28-week-old SHR rats （Spontaneously Hypertensive rats） and WKY （Wistar-Kyoto rats） of the same age were selected as experimental animals. Cardiac load was assessed by calculating the cardiac weight index. Collagen fibers and elastic fibers were isolated from the rat thoracic aorta by hot alkali method, and their content was determined by biochemical analysis. In addition, pathological evaluation of tissue sections of the left ventricle and thoracic aorta were performed by H&E staining, Sirius red staining, and lichen red staining. Western blotting was used to determine the expression level of sFRP2 protein in cardiac tissues. Results Compared with WKY rats, the heart weight index of SHR rats increased significantly （P<0.001）, and the results of biochemical analysis and staining of pathological sections showed that the content of collagen fibers in the aorta in the SHR group was higher than that in the WKY group, while the content of elastic fibers was lower, but the difference did not have statistical significance. The content of collagen fibers in the heart of the SHR group was significantly higher than that in the WKY group （P<0.01）. Western blotting showed that there was no significant difference in the expression level of sFRP2 protein in heart tissues between the two groups. Conclusion The remodeling of cardiac and aortic tissues in a rat model of spontaneous hypertension may involve complex molecular mechanisms, not just changes in the content of collagen fibers and elastic fibers. The detailed mechanism of the progression of spontaneous hypertension and target organs damage still need further investigation.
