Effects of peiminine B on Streptococcus pneumoniae-induced alveolar epithelial cell injury and its mechanism
- VernacularTitle:贝母素乙对肺炎链球菌诱导肺泡上皮细胞损伤的影响及机制
- Author:
Rui ZHANG
1
;
Cuihong LI
1
;
Youqin WANG
1
;
Junyan GUAN
2
Author Information
1. Dept. of Pediatrics，Suizhou Hospital Affiliated to Hubei Medical College （Suizhou Central Hospital），Hubei Suizhou 441300，China
2. Dept. of Pediatrics，Xiangyang Central Hospital Affiliated to Hubei University of Arts and Sciences，Hubei Xiangyang 441021，China
- Publication Type:Journal Article
- Keywords:
peiminine B;
Streptococcus pneumoniae;
alveolar epithelial cells;
Rac1/Akt/NF-κB signaling pathway
- From:
China Pharmacy
2025;36(7):820-825
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To investigate the effects of peiminine B （PEI） on Streptococcus pneumoniae （SP）-induced alveolar epithelial cell injury by regulating the Ras-related C3 botulinum toxin substrate 1 in nucleus accumbens （Rac1）/protein kinase B （Akt）/nuclear factor κB （NF-κB） signaling pathway. METHODS Human alveolar epithelial cells （HPAEpiC） were taken and randomly divided into the Control group， SP group （1×108 cfu/mL SP bacterial solution）， low-， medium-， and high-concentration PEI groups （1×108 cfu/mL SP bacterial solution+0.05， 0.10， 0.20 mmol/L PEI）， and high-concentration PEI+Akt activator group （P-H+SC79 group， 1×108 cfu/mL SP bacterial solution+0.20 mmol/L PEI+10 μmol/L SC79）. Except for the Control group， the other groups of cells were treated with SP bacterial solution and/or corresponding drug solution. After 24 h of treatment， the levels of inflammatory factors （interleukin-6， -18， -1β） in the supernatant solution， the contents of oxidative stress indexes [lactate dehydrogenase （LDH）， reactive oxygen species （ROS） and superoxide dismutase （SOD）]， apoptosis rate， as well as the expressions of proliferation/apoptosis-related proteins [cyclin-dependent kinase 1 （CDK1）， B cell lymphoma-2 related X protein （Bax）] and pathway-related proteins （Rac1， Akt， phosphorylated Akt， NF-κB and phosphorylated NF-κB） were detected in each group. RESULTS Compared with the Control group， the levels of inflammatory factors in supernatant solution， LDH and ROS contents， apoptosis rate， the protein expressions of Bax and Rac1 and the phosphorylation levels of Akt and NF-κB in the SP group were significantly increased or up-regulated， while SOD content and the protein expression of CDK1 were significantly decreased or down-regulated （P＜0.05）. Compared with the SP group， the above indexes in PEI groups were significantly improved in a concentration-dependent manner （P＜0.05）. SC79 could significantly reverse the improvement effect of the high concentration of PEI （P＜0.05）. CONCLUSIONS PEI can alleviate SP-induced inflammation and oxidative stress damage of alveolar epithelial cells and inhibit apoptosis， which may be achieved by inhibiting Rac1/Akt/NF-κB signaling pathway.
