Study on secondary metabolites of <italic>Penicillium expansum</italic> GY618 and their tyrosinase inhibitory activities

Fei-yu YIN; Sheng LIANG; Qian-heng ZHU; Feng-hua YUAN; Hao HUANG; Hui-ling WEN

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Study on secondary metabolites of Penicillium expansum GY618 and their tyrosinase inhibitory activities

VernacularTitle:扩展青霉Penicillium expansum GY618的次生代谢产物及抑制酪氨酸酶活性研究
Author: Fei-yu YIN ¹ ; Sheng LIANG ² ; Qian-heng ZHU ³ ; Feng-hua YUAN ² ; Hao HUANG ³ ; Hui-ling WEN ³
Author Information

1. School of Rehabilitation, Gannan Medical University, Ganzhou 341000, China
2. School of Basic Medicine, Gannan Medical University, Ganzhou 341000, China
3. School of Pharmacy, Gannan Medical University, Ganzhou 341000, China
Publication Type:Research Article
Keywords: italic>Penicillium expansum; rice fermentation; secondary metabolite; structural identification; tyrosinase inhibitory activity
From: Acta Pharmaceutica Sinica 2025;60(2):427-433
CountryChina
Language:Chinese
Abstract: Twelve compounds were isolated from the rice fermentation extracts of Penicillium expansum GY618 by silica column chromatography, Sephadex gel column chromatography, ODS column chromatography and semi preparative HPLC methods. They were determined as 11-hydroxyl-penicitrinone F (1), penicitrinone F (2), betulin (3), erythrodiol (4), ergosterol (5), ergost-5α,8α-epidioxy-6,22-dien-3β-ol (6), (5α,8α-epidioxy-(22E,24R)-ergosta-6,9(11),22-trien-3β-ol) (7), 5α,8α-epidioxy-(22E,24R)-23-methylergosta-6,22-dien-3β-ol (8), 5α,8α-epidioxy- 23,24(R)-dimethylcholesta-6,9(11),22-trien-3β-ol (9), dankasterone A (10), (17R)-4-hydroxy-17-methylincisterol (11) and ergosta-4,6,8(14),22-tetraen-3-one (12), through mass spectrometer, nuclear magnetic resonance (NMR) and comparison with the literature. Compound 1 was a new compound and compounds 2-4, 6-12 were isolated from Penicillium expansum fungus for the first time. The tyrosinase inhibitory activity experiment showed that compounds 1, 3 and 12 showed certain inhibitory activity against tyrosinase with IC₅₀ values of (75 ± 9), (69 ± 8) and (64 ± 2) μmol·L^-1, respectively. The IC₅₀ of other compounds were all greater than 100 μmol·L^-1, while IC₅₀ of the positive control kojic acid was (46 ± 4) μmol·L^-1.