Preparation of new hydrogels and their synergistic effects of immunochemotherapy
10.16438/j.0513-4870.2024-0658
- VernacularTitle:新型水凝胶的制备及其发挥免疫化疗协同作用的研究
- Author:
Wen-wen YAN
1
;
Yan-long ZHANG
2
;
Ming-hui CAO
2
;
Zheng-han LIU
2
;
Hong LEI
3
;
Xiang-qian JIA
2
Author Information
1. Ministry of Education & Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region & Key Laboratory of Molecular Biology, Engineering Research Center of Agricultural Microbiology Technology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, China; Ministry of Education & Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region & Key Laboratory of Microbiology, Engineering Research Center of Agricultural Microbiology Technology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, China
2. Ministry of Education & Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region & Key Laboratory of Microbiology, Engineering Research Center of Agricultural Microbiology Technology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, China
3. Ministry of Education & Heilongjiang Provincial Key Laboratory of Ecological Restoration and Resource Utilization for Cold Region & Key Laboratory of Molecular Biology, Engineering Research Center of Agricultural Microbiology Technology, College of Heilongjiang Province & School of Life Sciences, Heilongjiang University, Harbin 150080, China
- Publication Type:Research Article
- Keywords:
hydrogel composite system;
tumor vaccine;
DC2.4 dendritic cell;
tumor cell;
immuno-combination chemotherapy
- From:
Acta Pharmaceutica Sinica
2025;60(2):479-487
- CountryChina
- Language:Chinese
-
Abstract:
In recent years, cancer treatment methods and means are becoming more and more diversified, and single treatment methods often have limited efficacy, while the synergistic effect of immunity combined with chemotherapy can inhibit tumor growth more effectively. Based on this, we constructed a sodium alginate hydrogel composite system loaded with chemotherapeutic agents and tumor vaccines (named SA-DOX-NA) with a view to the combined use of chemotherapeutic agents and tumor vaccines. Firstly, the tumor vaccine (named NA) degradable under acidic conditions was constructed by in situ polymerization using chicken ovalbumin (OVA), acrylamide (AAM) and 2-(dimethylamino) ethyl methacrylate (DMAEMA) monomer. Then a hydrogel composite system SA-DOX-NA co-loaded with chemotherapeutic drug doxorubicin (DOX) and NA was prepared using sodium alginate as a matrix. The results showed that SA-DOX-NA had good in situ gel-forming ability and formed a mesh structure that could realize the co-loading of DOX and NA as well as the slow drug release. The electron microscopy results showed that SA-DOX-NA had good in situ gel-forming ability with good internal connectivity, and the three-dimensional mesh structure could realize the co-loading of DOX and NA as well as the slow drug release. The antitumor and immunomodulatory results showed that SA-DOX-NA both effectively inhibited the growth of tumor cells and efficiently promoted the proliferation and activation of DC2.4 dendritic cells without additional adjuvant. In summary, SA-DOX-NA exerts the dual efficacy of chemotherapy and immunotherapy for tumor treatment, and has a good application prospect in local tumor treatment.
