Identification of monoclonal antibodies against spike protein of severe fever with thrombocytopenia syndrome virus based on immune repertoire sequencing
10.13200/j.cnki.cjb.004400
- VernacularTitle:基于免疫组库测序筛选发热伴血小板减少综合征病毒刺突蛋白单克隆抗体
- Author:
Wenjun ZHENG
1
Author Information
1. Department of Pathogen Biology,School of Clinical and Basic Medical Sciences,Shandong First Medical University & Shandong Academy of Medical Sciences
- Publication Type:Journal Article
- From:
Chinese Journal of Biologicals
2025;38(01):14-21
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain monoclonal antibodies against the glycoprotein Gn of severe fever with thrombocytopenia syndrome virus(SFTSV) based on immune repertoire sequencing and identify the biological characteristics.Methods Using purified SFTSV-Gn as bait, virus-specific B cells were captured from B cell library of immunized female BALB/c mice. Based on 10 × immune repertoire sequencing and bioinformatics analysis, the antibody sequences with potential binding activity were selected and constructed into eukaryotic expression vector pcDNA3.4. The monoclonal antibodies were expressed and purified, and identified for the biological activity.Results Fourteen antibodies were selected from 3 544 captured B cells, and the functional verification showed that 12 antibodies could bind to SFTSV-Gn glycoprotein, among which C9 and 9/10 antibodies had higher binding ability. Western blot, indirect immunofluorescence assay(IFA) and flow cytometry proved that they specifically recognized SFTSV, and the effective concentration was as low as 2 μg/mL.Conclusion The monoclonal antibodies against SFTSV-Gn glycoprotein were successfully obtained, which lays a foundation for the development of rapid diagnostic reagents for SFTSV.
- Full text:2025032216034820699基于免疫组库测序筛选发热伴...合征病毒刺突蛋白单克隆抗体_郑文君.pdf
