Effects of Danggui Buxue Decoction on myocardial ferroptosis in a rat model of heart failure with preserved ejection fraction
10.3969/j.issn.1001-1528.2024.11.013
- VernacularTitle:当归补血汤对射血分数保留型心力衰竭大鼠心肌组织铁死亡的影响
- Author:
Ting JIA
1
;
Dan QI
;
Jing MA
;
Huan LIU
;
Jing ZHAO
;
Song-Yan XUE
;
Fang-Yuan WANG
Author Information
1. 空军军医大学第一附属医院中医科,陕西 西安 710032
- Keywords:
Danggui Buxue Decoction;
heart failure with preserved ejection fraction;
myocardium;
ferroptosis;
GPX4;
FTH1;
xCT
- From:
Chinese Traditional Patent Medicine
2024;46(11):3629-3635
- CountryChina
- Language:Chinese
-
Abstract:
AIM To observe the effects of Danggui Buxue Decoction on myocardial ferroptosis in a rat model of heart failure with preserved ejection fraction(HFpEF).METHODS The rats were randomly divided into the control group,the model group and the Danggui Buxue Decoction group.After 8 weeks feeding of 8%high-salt diet in the model and trial groups to induce the rats into HFpEFd models,rats of the Danggui Buxue Decoction group were given 4 g/kg Danggui Buxue Decoction once daily for 4 weeks,in contrast to those of the control group given 12 weeks feeding of 0.3%low-salt diet.The rats had their left ventricular ejection fraction(LVEF),left ventricular fraction shortening(LVFS),left ventricular end-diastolic diameter(LVIDD),and end-diastolic left ventricular posterior wall thickness(LVPWd)detected by echocardiography;their pathological changes of myocardial tissue by HE and Masson staining;their myocardial mitochondrial morphology observed by transmission electron microscopy;their serum BNP,NT-proBNP levels,myocardial tissue Fe3+levels,and their levels of ROS,MDA,LPO and GSH,and SOD activity in serum and myocardium detected by ELISA method;and their myocardial expressions of ferroptosis marker proteins GPX4,FTH1 and xCT detected by immunofluorescence staining and Western blot method.RESULTS Compared with the model group,the Danggui Buxuetang group displayed increased LVEF,LVFS(P＜0.01);decreased LVIDD,LVPWd(P＜0.05,P＜0.01);decreased serum BNP,NT-proBNP levels(P＜0.01);decreased myocardial Fe3+level(P＜0.01);decreased MDA,ROS and LPO levels in serum and myocardium(P＜0.01);increased GSH level and SOD activity(P＜0.01);and increased expressions of myocardial ferroptosis related protein GPX4,FTH1 and xCT(P＜0.05,P＜0.01).CONCLUSION Danggui Buxue Decoction protects the cardiac function of the rat model of HFpEF through inhibiting the occurrence of myocardial ferroptosis and reducing the myocardial oxidative stress level as well.
