Protective effects of Shiwei Ruxiang Powder on renal injury in rats with gouty nephritis by regulating mitochondrial autophagy
10.3969/j.issn.1001-1528.2024.09.012
- VernacularTitle:十味乳香散通过调控线粒体自噬对痛风性肾炎大鼠肾损伤的保护作用
- Author:
Yan-Rong ZHU
1
;
He-Bing XIE
;
Chun-Xiang GONG
;
Jie-Nan ZHAO
;
Zhi-Bing YUAN
Author Information
1. 安徽中医药大学药学院,安徽 合肥 230012;南通市海门长三角药物高等研究院,江苏 南通 226133
- Keywords:
Shiwei Ruxiang Powder;
gouty nephropathy;
oxidative stress;
inflammation;
mitophagy
- From:
Chinese Traditional Patent Medicine
2024;46(9):2923-2930
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the renal protective effects of Shiwei Ruxiang Powder on gouty nephritis in rats based on mitophagy.METHODS Rats were randomly divided into the blank group,the model group,the low-dose,medium-dose,and high-dose Shiwei Ruxiang Powder groups(200,400,800 mg/kg)and allopurinol group(10 mg/kg).The rat model of gouty nephropathy was established by gavage of potassium oxyzinate(750 mg/kg)and uric acid(300 mg/kg).The rats had their levels of UA,SCr,BUN,XOD,SOD,MDA,ROS measured by automatic biochemical analyzer,ELISA and chemical fluorescence method;their renal pathological changes observed by HE staining;their apoptosis of renal tissue cells observed by TUNEL staining;and their mRNA and protein expressions of IL-1β,TNF-α,Bax,Bcl-2,caspase-3,caspase-9,PINK1,Parkin and LC3-Ⅱ detected by RT-qPCR and Western blot.RESULTS Compared with the model group,Shiwei Ruxiang Powder groups displayed dose-dependently decreased serum levels of UA,BUN and SCr,renal deposition of urate crystal and apoptosis(P<0.05);decreased renal levels of ROS and inflammatory factors IL-1β and TNF-α(P<0.05);and increased renal expressions of mitochondrial autophagy-related proteins PINK1,Parkin and LC3-Ⅱ(P<0.01).CONCLUSION Shiwei Ruxiang Powder may relieve gouty kidney injury in rats by reducing the uric acid level,the renal oxidative stress and inflammatory response,and activating mitophagy pathway as well.
