Quality standard for Zhujieshen Formula Granules based on standard decoction
10.3969/j.issn.1001-1528.2024.09.004
- VernacularTitle:基于标准汤剂的竹节参配方颗粒质量标准研究
- Author:
Chen-Hui YE
1
;
Hai-Ming TANG
;
Cheng-Fu YUAN
;
Jia-Long GUO
;
Ji-Hong ZHANG
;
Ding YUAN
;
Yu-Min HE
Author Information
1. 三峡大学,国家中医药管理局药理科研三级实验室,湖北 宜昌 443002;三峡大学健康医学院,湖北 宜昌 443002
- Keywords:
Zhujieshen Formula Granules;
standard decoction;
ginsenoside Ro;
chikusetsu saponin Ⅳa;
HPLC characteristic chromatograms
- From:
Chinese Traditional Patent Medicine
2024;46(9):2863-2869
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish the quality standard of the zhujieshen Formula Granules based on standard decoction.METHODS The contents and transfer rates of ginsenoside Ro and chikusetsu saponin Ⅳa in standard decoction and formula granules were determined by HPLC,after which the transfer rates were calculated.The HPLC characteristic chromatograms of standard decoctions were established,after which cluster analysis and principal component analysis were adopted.Then the HPLC characteristic chromatograms of formula granules were established.RESULTS Nine common peaks were found in the HPLC characteristic chromatograms of seventeen batches of standard decoctions with the similarities of more than 0.9(except for S6,S12),which were clustered into two categories,and the accumulative variance contribution rate of three principal components reached 86.7％.The contents of ginsenoside Ro in three batches of formula granules were 83.1-88.6 mg/g,and the transfer rates were 53.1％-55.5％.The contents of chikusetsusaponin Ⅳa were 14.8-15.0 mg/g,and the transfer rates were 47.4％-48.1％.Nine common peaks were found in the HPLC characteristic chromatograms of three batches of formula granules with the similarities of 0.998,0.998 and 0.999,respectively.CONCLUSION This reasonable and reliable method can comprehensively evaluate the quality of Zhujieshen Formula Granules,and provide a reference for the quality control.
