Simultaneous content determination of eleven ginsenosides in Fukang Tablets by UPLC-MS/MS
10.3969/j.issn.1001-1528.2024.09.003
- VernacularTitle:UPLC-MS/MS法同时测定妇康片中11种人参皂苷的含量
- Author:
Si-Min XIE
1
;
Jun-Fei CHEN
;
Hao-Yu HUANG
;
Li-Hong GU
;
Jian-Ming LI
Author Information
1. 广州市药品检验所,国家药品监督管理局中成药质量评价重点实验室,广东 广州 510160
- Keywords:
Fukang Tablets;
ginsenosides;
Ginseng Radix et Rhizoma;
content determination;
UPLC-MS/MS
- From:
Chinese Traditional Patent Medicine
2024;46(9):2858-2863
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish a UPLC-MS/MS method for the simultaneous content determination of pseudoginsenoside F11 and ginsenosides Rf,Rg1,Re,Rb1,Rb2,Rb3,Rg2,Rg3,Rc,Rd.METHODS The analysis was performed on a 40℃ thermostatic Phenomenex Kinetex F5 column(2.1 mm×100 mm,1.7 μm),with the mobile phase comprising of acetonitrile-2 mmol/L ammonium formate flowing at 0.3 mL/min in a gradient elution manner,and electron spray ionization source was adopted in negative ion scanning with multiple reaction monitoring mode.RESULTS Eleven ginsenosides showed good linear relationships within their own ranges(r>0.9900),whose average recoveries were 97.40％-103.74％with the RSDs of 1.76％-3.48％.CONCLUSION This sensitive,practical and reproducible method can be used for the quality control of Ginseng Radix et Rhizoma in Fukang Tablets.
