Chemical constituents from Rhinacanthus nasutus and their in vitro antitumor and lipid-lowering activities
10.3969/j.issn.1001-1528.2024.08.023
- VernacularTitle:白鹤灵芝化学成分及其体外抗肿瘤与降脂活性研究
- Author:
Zhi-Qiang GONG
1
;
Yang XIAO
;
Sha HAN
;
Ying LIU
;
Jin QI
;
Yu HUO
;
Yan-Hong HUANG
;
Li-Fang YANG
Author Information
1. 广西中医药大学赛恩斯新医药学院,瑶医药学院,广西壮瑶药重点实验室,广西 南宁 530200;广西民族大学化学化工学院,广西林产化学与工程重点实验室,广西 南宁 530008
- Keywords:
Rhinacanthus nasutus(L.)Kurz;
chemical constituents;
isolation and identification;
anti-tumor;
lipid-lowering
- From:
Chinese Traditional Patent Medicine
2024;46(8):2630-2637
- CountryChina
- Language:Chinese
-
Abstract:
AIM To study the Chemical constituents from Rhinacanthus nasutus(L.)Kurz and their in vitro antitumor and lipid-lowering activities.METHODS The ethyl acetate fraction from R.nasutus was isolated and purified by normal phase,reverse phase silica gel column and semi-preparative HPLC,then the structures of obtained compounds were identified by physicochemical properties and spectral data.The in vitro anti-tumor activity was determined by MTT assay,and the in vitro lipid-lowering activity was evaluated by oleic acid-induced HepG2 high-fat cell model.RESULTS Twenty compounds were isolated and identified as rhinacanthin A(1),rhinacanthin B(2),rhinacanthin C(3),rhinacanthin D(4),rhinacanthin E(5),rhinacanthin M(6),rhinacanthin N(7),rhinacanthin Q(8),rhinacanthin T(9),rhinacanthone(10),β-sitosterol(11),gallic acid(12),vanillic acid(13),syringic acid(14),lapachol(15),umbelliferone(16),sambucunlin A(17),17α,21-dihydroxy-1,4-pregnadiene-3,11,20-trione,21-acetate(18),1,8-dihydroxyanthraquinone(19),2,6-dimethoxy-1,4-benzoquinone(20).Compounds 3 and 7 inhibited the proliferation of HepG2,Hela,A549 and H22 tumor cells with IC50 values of(0.66±0.17)-(3.22±0.49)μmol/L in a time-and concentration-dependent manner.Compounds 3,7 and 9 had a lipid clearance rate of more than 50%in oleic acid-induced HepG2 high-fat cells.CONCLUSION Compounds 16-20 are isolated from this plant for the first time.Compounds 3 and 7 have good in vitro anti-tumor activities,and 3,7,9 have good in vitro lipid-lowering activities.
