Simultaneous content determination of fifteen constituents in Binglang Sixiao Pills by HPLC
10.3969/j.issn.1001-1528.2024.08.001
- VernacularTitle:HPLC法同时测定槟榔四消丸中15种成分的含量
- Author:
Yuan WANG
1
;
Yuan-Shuo YANG
;
Si-Zhi LI
;
Jia-Meng ZHAO
Author Information
1. 中国人民解放军联勤保障部队第九八三医院药剂科,天津 300142
- Keywords:
Binglang Sixiao Pills;
chemical constituents;
content determination;
HPLC;
cluster analysis;
principal component analysis
- From:
Chinese Traditional Patent Medicine
2024;46(8):2493-2498
- CountryChina
- Language:Chinese
-
Abstract:
AIM To establish an HPLC method for the simultaneous content determination of gallic acid,catechin,epicatechin,rhein 8-O-β-D-glucoside,chlorogenic acid,chrysophanol 8-O-β-D-glucoside,emodin-8-O-β-D-glucoside,emodin-3-methyl ether-8-O-β-D-glucoside,aloe emodin,rhein,cyperotundone,luteolin,α-cyperone,emodin,chrysophanol and emodin-3-methyl ether in Binglang Sixiao Pills.METHODS The analysis was performed on a 30℃thermostatic Kromasil C18 column(250 mm×4.6 mm,5 μm),with the mobile phase comprising of methanol-0.1%formic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 260 nm.Subsequently,cluster analysis and principal component analysis were made.RESULTS Fifteen constituents showed good linear relationships within their own ranges(r＞0.999 0),whose average recoveries were 96.00%-101.00%%with the RSDs of 0.50%-1.50%.Various batches of samples were clustered into two categories,and four principal components demonstrated the accumulative variance contribution rate of 90.004%.CONCLUSION This simple,accurate and reproducible method can be used for the quality control of Binglang Sixiao Pills.
