Effects of different processes on the overall quality of Yanggan Jiangcao Mixture
10.3969/j.issn.1001-1528.2024.07.006
- VernacularTitle:不同工艺对养肝降草合剂整体质量的影响
- Author:
Yu-Xin LIU
1
;
Ya-Ping ZHU
;
Li-Yuan TIAN
;
Lei WU
Author Information
1. 南京中医药大学附属医院,江苏 南京 210029;南京中医药大学第一临床医学院,江苏 南京 210029
- Keywords:
Yanggan Jiangcao Mixture;
extraction process;
concentration process;
centrifugation process;
sterilization process;
HPLC fingerprints;
content determination
- From:
Chinese Traditional Patent Medicine
2024;46(7):2157-2163
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effects of extraction,concentration,centrifugation,sterilization processes on overall quality of Yanggan Jiangcao Mixture.METHODS The analysis was performed on a 20℃ thermostatic Phenomenex Luna C18column(250 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1% phosphoric acid flowing at 1 mL/min in a gradient elution manner.The HPLC fingerprints were established,after which the contents of salidroside,geniposide,forsythoside A,specnuezhenide,glycyrrhizic acid and atractylenolideⅢ,along with the calculation of their transfer rates.RESULTS There were twenty common peaks in the fingerprints for fifteen batches of samples under different processes with the similarities of more than 0.950.Six constituents showed good linear relationships within their own ranges(r≥0.999 0),whose average recoveries were 96.96%-106.69% with the RSDs of 0.97%-2.87%,and the transfer rates and total transfer rates were more than 80% and more than 90%,respectively.CONCLUSION Yanggan Jiangcao Mixture demonstrates good stability under different processes.
