Effects of Compound Dihuang Granules on biological function of dopaminergic neurons in rats with Parkinson's disease of Pattern of Yin-Deficiency with Stirring Wind
10.3969/j.issn.1001-1528.2024.06.009
- VernacularTitle:复方地黄颗粒对帕金森病阴虚动风证大鼠多巴胺能神经元生物学功能的影响
- Author:
Xing-Ling WANG
1
;
Jian-Qing LIANG
;
Guang-Jie SUN
;
Zi-Wei LÜ
;
Jian-Cheng HE
Author Information
1. 甘肃中医药大学基础医学院,甘肃兰州 730000
- Keywords:
Compound Dihuang Granules;
Parkinson's disease;
Pattern of Yin-Deficiency with Stirring Wind;
DJ-1;
IP3R;
GRP75;
VDAC1
- From:
Chinese Traditional Patent Medicine
2024;46(6):1828-1835
- CountryChina
- Language:Chinese
-
Abstract:
AIM To investigate the effects of Compound Dihuang Granules on biological function of dopaminergic neurons in rats with Parkinson's disease(PD)of Pattern of Yin-Deficiency with Stirring Wind.METHODS The PD animal model of Pattern of Yin-Deficiency with Stirring Wind established by intracerebral injection of 6-hydroxydopamine(6-OHDA)were randomly divided into the model group,the Midoba group(150 mg/kg)and the low,medium and high dose Compound Dihuang Granules groups(1.75,3.5 and 7 g/kg)for corresponding drug intervention,in contrast to the normal group,the sham operation group and the model group underwent 28-day normal saline administration.The rats had their general condition and neuroethology observed;their pathological changes of substantia nigra observed by HE staining;their mitochondrial structure of dopaminergic neurons in the damaged substantia nigra observed by transmission electron microscopy;and their expressions of DJ-1,IP3R,GRP75 and VDAC1 detected by immunohistochemistry,Western blot and RT-qPCR.RESULTS Compared with the normal group and sham operation group,the model group displayed increased rotational behavirors(P＜0.01),decreased swimming time score(P＜0.01),decreased hanging time(P＜0.01),decreased number of neurons in substantia nigra but more neurons with morphological damage,mitochondrial swelling and degeneration,mitochondrial crista disappearance,and decreased expressions of DJ-1,IP3R,GRP75 and VDA C1 mRNA and protein in the injured side(P＜0.01).Compared with the model group,the Midoba group and the Compound Dihuang Granules groups demonstrated less rotational behavirors(P＜0.01),higher swimming time score(P＜0.01),longer hanging time(P＜0.01),less damage to mitochondrial morphology and structure,and higher expressions of DJ-1,IP3R,GRP75 and VDA C1 mRNA and protein in the injured substantia nigra(P＜0.05,P＜0.01).The high-dose Compound Dihuang Granules presented equivalent efficacy to that of Midoba.CONCLUSION Compound Dihuang Granules may promote endoplasmic reticulum-mitochondria homeostasis,reduce mitochondrial damage and maintain the biological function of dopaminergic neurons by regulating the expressions of DJ-1,IP3R,GRP75 and VDAC1.
