Analysis of metabolites of nobiletin in rats in vivo based on characteristic ions
10.3969/j.issn.1001-1528.2024.06.005
- VernacularTitle:基于特征离子分析川陈皮素在大鼠体内代谢产物
- Author:
Zhe LI
1
;
Yu-Qing WANG
;
Dong-Xue WU
;
Shuang-Feng LI
;
Ya-Nan LI
;
Shao-Ping WANG
;
Jia-Yu ZHANG
;
Long DAI
Author Information
1. 山东中医药大学药学院,山东济南 250300;滨州医学院药学院,山东烟台 264003
- Keywords:
nobiletin;
metabolites;
characteristic ions;
UHPLC-HRMS
- From:
Chinese Traditional Patent Medicine
2024;46(6):1800-1809
- CountryChina
- Language:Chinese
-
Abstract:
AIM To analyze the metabolites of nobiletin in rats in vivo based on characteristic ions.METHODS Ten rats were assigned into administration group and control group,and given intragastric administration of the 0.5％CMC-Na suspension of nobiletin(250 mg/kg)and 0.5％CMC-Na solution,respectively,after which plasma,urine and feces were collected,solid phase extraction method was adopted in pretreatment,UHPLC-HRMS analysis was performed.The candidate metabolites were systematically described according to diagnostic product ions,chromatographic retention time,accurate molecular weight and neutral loss fragments,after which accurate metabolites were obtained in the established metabolite data set with-CH3(m/z 15)characteristic ions as a baits.RESULTS A total of 64 metabolites were identified,whose main metabolic pathways were glucuronidation,sulfation,hydrogenation and their compound reactions.CONCLUSION This experiment elucidates the metabolites of nobiletin in rats in vivo,which provides a new reference for its further development.
