Extraction process optimization and quality standard establishment for Jigen Standard Decoction
10.3969/j.issn.1001-1528.2024.06.001
- VernacularTitle:鸡根标准汤剂提取工艺优化及其质量标准建立
- Author:
Guo-Chun YANG
1
;
Ya-Fang YANG
;
Su-E XU
;
Jin KE
;
Ling-Yun CHEN
;
An-Guo HOU
;
Wen-Bin JIN
Author Information
1. 云南中医药大学中药学院,云南昆明 650500
- Keywords:
Jigen Standard Decoction;
extraction process;
quality standard;
response surface method;
HPLC characteristic chromatograms;
cluster analysis;
principal component analysis;
orthogonal partial least squares discriminant analysis
- From:
Chinese Traditional Patent Medicine
2024;46(6):1773-1781
- CountryChina
- Language:Chinese
-
Abstract:
AIM To optimize the extraction process for Jigen Standard Decoction,and to establish its quality standard.METHODS With soaking time,water addition and first decoction time as influencing factors,comprehensive score for 3,6'-disinapoyl sucrose content and yield rate as an evaluation index,the extraction process was optimized by response surface method on the basis of single factor test.The content and transfer rate of 3,6'-dimustayl sucrose were determined,after which HPLC characteristic chromatograms were established,cluster analysis,principal component analysis and orthogonal partial least squares discriminant analysis were performed.RESULTS The optimal conditions were determined to be 60 min for soaking time,(12+11)times for water addition,and(47+20)min for decoction time,the comprehensive score was 97.98.Fifteen batches of standard decoctions demonstrated the average yield rate and transfer rate of 14.182％and 20.468％,respectively,whose characteristic chromatograms existed six common peaks with the similarities of more than 0.9(except for S4,S8).Various batches of standard decoctions were clustered into two types,three principal components displayed the acumulative variance contribution rate of 91.4％,peaks 2,6 were quality markers.CONCLUSION This precise,stable and reproducible method can be used for the preparation and quality control of Jigen Standard Decoction.
