Quality control method improvement of Zanthoxylum nitidum
10.3969/j.issn.1001-1528.2024.05.004
- VernacularTitle:两面针质量控制方法改进
- Author:
Ding-Bang AN
1
;
Qi-Yan LIN
;
Xiang ZHAO
;
Xue-Mei CHENG
;
Chang-Hong WANG
Author Information
1. 上海中医药大学中药研究所,上海中药标准化研究中心,中药标准化教育部重点实验室,上海市复方中药重点实验室,上海 201203
- Keywords:
Zanthoxylum nitidum(Roxb.)DC.;
qualitative identification;
fingerprints;
content determination;
TLC;
HPLC
- From:
Chinese Traditional Patent Medicine
2024;46(5):1440-1446
- CountryChina
- Language:Chinese
-
Abstract:
AIM To improve the quality control method of Zanthoxylum nitidum(Roxb.)DC.METHODS In the TLC qualitative identification of nitidine chloride,chelerythrine and toddalolactone,the analysis was performed on silica gel GF254 TLC plate,chloroform-methanol-ammonia(30∶1∶0.1)was taken as a developing agent.The HPLC fingerprints for Z.nitidum and its adulterants were established.In the HPLC content determination of magnoflorine,nitidine chloride and chelythrine,the analysis was performed on a 30℃ thermostatic Diamonsil Plus column(250 mm×4.6 mm,5 μm),with the mobile phase comprising of acetonitrile-0.1%trifluoroacetic acid flowing at 1.0 mL/min in a gradient elution manner,and the detection wavelength was set at 273 nm.RESULTS The clear TLC plots demonstrated good separation.The similarities of fingerprints for eighteen batches of medicinal materials were 0.484-0.983,Z.nitidum and its adulterants were effectively distinguished.Three constituents showed good linear relationships within their own ranges(R2=1.000 0),whose average recoveries were 98.9%-103.3%with the RSDs of 1.17%-1.96%.CONCLUSION This simple and reproducible can provide a new method for the quality control of Z.nitidum.
