Sperm ultrastructure in patients with partial globozoospermia
10.13263/j.cnki.nja.2024.10.001
- VernacularTitle:部分型圆头精子症患者精子超微结构研究
- Author:
Hao-Ran LIU
1
;
Liu YANG
;
Hui TIAN
;
Yuan-Yuan JI
;
Xiao-Fang HAN
;
Xiang HUANG
Author Information
1. 山西医科大学附属山西省人民医院中心实验室,山西太原 030012
- Keywords:
round-headed spermatozoa;
sperm morphology;
ultrastructure;
transmission electron microscopy;
intracytoplasmic single sperm injection
- From:
National Journal of Andrology
2024;30(10):867-871
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the morphological and ultrastructural differences between normal and round-headed sperm and the outcomes of assisted reproduction in patients with partial globozoospermia.Methods:We collected clinical information and semen samples from 6 cases of partial globozoospermia confirmed in our hosptials from March 2023 to March 2024,performed Diff-Quik staining of the samples,observed the sperm morphology under the light microscope and transmission electron microscope,compared the ultrastructures between round-headed and normal spermatozoa,and analyzed the clinical outcomes of assisted reproduction in the pa-tients.Results:Under the light microscope,the the round-headed sperm had no acrosomal structure,some with tail abnormalities;while under the transmission electron microscope,they exhibited dissolved plasma membrane,acrosomal absence,small acrosomes,nucleoplasmic granulation,huge central vacuoles,swelling,absence or irregular arrangement of mitochondria in the neck,and disor-ganized or missed 9+2 microtubules in the tail.All the 6 patients underwent intracytoplasmic sperm injection(ICSI),and 1 of them achieved pregnancy.Conclusion:The sperm organelle is morphologically damaged and ultrastructurally abnormal in some patients with partial globozoospermia,which may be a key factor for their infertility,and ICSI can help some of them to achieve fertility.
