Therapeutic effect of Lingze Tablets on benign prostatic hyperplasia in rats:An exploration based on the VEGFA/TNF/IL-6 signaling pathway
10.13263/j.cnki.nja.2024.07.009
- VernacularTitle:基于VEGFA/TNF/IL-6信号通路探讨灵泽片对良性前列腺增生大鼠的治疗作用
- Author:
Hua-Nan ZHANG
1
;
Song SUN
;
Jun-Long FENG
;
Yi-Chen LIU
;
Hai-Song LI
;
Jin-Sheng WANG
;
Bin WANG
Author Information
1. 北京中医药大学东直门医院,北京 100700
- Keywords:
Lingze Tablets;
benign prostatic hyperplasia;
VEGFA/TNF/IL-6 signaling pathway;
cell proliferation;
inflammato-ry response
- From:
National Journal of Andrology
2024;30(7):634-639
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the mechanism of Lingze Tablets(LZT)acting on BPH in rats based on the VEGFA/TNF/IL-6 signaling pathway.Methods:We equally randomized 30 SPF SD male rats into five groups,normal control,BPH model con-trol,low-dose LZT,medium-dose LZT and high-dose LZT,and established a BPH model in the latter four groups by induction with non-castrate testosterone propionate.After the modeling,we treated the rats in the normal and model groups by intragastrical adminis-tration of physiological saline,and those in the latter three groups with low-,medium-,and high-dose LZT respectively,all for 28 suc-cessive days.Then we collected the prostate tissue from the animals for observation of the changes in the prostatic indexes and histo-morphology,detected the expressions of the proteins related to the VEGFA/TNF/IL-6 signaling pathway,and compared the data ob-tained among different groups.Results:Compared with the normal controls,the rats in the model control group showed significant pros-tatic hyperplasia,markedly increased prostatic index([0.84±0.01]g,P<0.05),thickness of the prostatic epithelia and infiltra-tion of the luminal area,and dramatically up-regulated protein expressions of VEGFA(0.60±0.02,P<0.05),TNF(0.76±0.02,P<0.05)and IL-6(0.64±0.02,P<0.05).In comparison with the model controls,the rats in the low-,medium-and high-dose LZT groups exhibited significantly decreased prostatic indexes([0.76±0.02]g,[0.58±0.02]g and[0.52 0.01]g,all P<0.05),improved prostatic histomorphology,and down-regulated expressions of VEGFA(0.45±0.01,0.35±0.01 and 0.31±0.02,all P<0.05),TNF(0.45±0.01,0.33±0.01 and 0.27±0.01,all P<0.01)and IL-6(0.44±0.01,0.36±0.01 and 0.30±0.01,all P<0.01)in a dose-dependent manner.Conclusion:LZT produces therapeutic effect on BPH by negatively regulating the VEGFA/TNF/IL-6 signaling pathway,reducing the expression levels of VEGFA,TNF and IL-6 pro-teins,and regulating cell proliferation,apoptosis and inflammatory response.
