Expression of Zfx in mouse testicular spermatogenic cells
10.13263/j.cnki.nja.2023.05.001
- VernacularTitle:Zfx在小鼠睾丸生精细胞中的表达
- Author:
Xiang-Zu WANG
1
;
Ji-Feng XI
;
Yi-Long ZHAO
Author Information
1. 新疆农业职业技术学院,新疆昌吉 831100;石河子大学,新疆石河子 832003
- Keywords:
Zfx;
mouse testis;
spermatogenic cells;
cell separation;
spermatogenesis
- From:
National Journal of Andrology
2023;29(5):387-392
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the expression of Zfx gene in spermatogenic cells.Methods:The testes of d6,d8,dl7 and adult mice were collected,single cell suspension was prepared by combinatorial enzyme digestion,spermatogenic cells were isola-ted by BSA density gradient method,and Zfx expression was detected by real-time quantitative polymerase chain reaction(qRT-PCR)and Western Blot(WB).Results:Single cell suspension prepared by combination enzyme digestion method and density gradient method laid with BSA can obtain various types of spermatogenic cells with purity＞85％;The expression level of the Zfx gene is low in primitive type A spermatogonia,type A spermatogonia,and type B spermatogonia,whereas it is high in preleptotene spermatocytes,pachytene spermatocytes,and round spermatid cells.It is not expressed in elongating spermatids and mature sperm.Conclusion:Zfx gene exhibits periodic expression in various levels of spermatogenic cells and may be an important transcription factor involved in regulating meiosis in spermatogenic cells.
