Copper starvation induces autophagy-mediated EZH2 degradation to enhance anti-tu-mor immunity in oral squamous cell carcinoma
10.12354/j.issn.1000-8179.2024.20240251
- VernacularTitle:铜饥饿诱导自噬介导EZH2降解增强口腔鳞状细胞癌抗肿瘤免疫
- Author:
Lin XIAOHU
1
;
Zhao ZHANG
;
Yu ZHONGLIN
;
Cao WEI
Author Information
1. 上海交通大学医学院附属第九人民医院口腔颌面头颈肿瘤科,上海交通大学口腔医学院,国家口腔医学中心,国家口腔疾病临床医学研究中心,上海市口腔医学重点实验室,上海市口腔医学研究所(上海市 200001)
- Keywords:
oral squamous cell carcinoma(OSCC);
copper starvation;
SLC31A1;
EZH2;
autophagy
- From:
Chinese Journal of Clinical Oncology
2024;51(5):224-230
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To examined the role of copper starvation in oral squamous cell carcinoma(OSCC),along with the molecular functions and mechanisms of SLC31A1 in these cells.Methods:Initially,SLC31A1 was silenced to induce a copper starvation environment.Sub-sequently,we evaluated the effects of copper starvation on oral squamous cell carcinoma cells using the CCK-8 assay,cell scratch assay,and subcutaneous tumor formation in nude mice.In addition,changes in autophagy and EZH2 expression levels in oral squamous cell carcinoma cells were detected after SLC31A1 silencing.Lactate dehydrogenase activity was assayed to determine its impact on natural killer(NK)cell cytotoxicity after SLC31A1 silencing.Results:Silencing of SLC31A1 significantly inhibited the proliferation,migration,and subcutaneous tu-mor formation ability of oral squamous cell carcinoma cells.Furthermore,silencing SLC31A1 mediated EZH2 degradation and increased NK cell infiltration.Conclusions:Copper starvation can regulate the proliferation,migration,and subcutaneous tumor formation ability of oral squamous cell carcinoma and increase NK cell infiltration by modulating EZH2 expression.
