Determination of vanillin in lysine and vitamin B12 granules by HPLC

VernacularTitle:HPLC法测定赖氨酸维B12颗粒中香草醛的含量
Author: Haiyan TIAN ¹ ; Juan LIU ; Weibin LIU ; Honghai LIU
Author Information

1. 德州市食品药品检验检测中心,山东德州 253000
Keywords: lysine and vitamin B12 granules; vanillin; HPLC method; pharmaceutical excipient; flavoring agent
From: Drug Standards of China 2024;25(5):517-520
CountryChina
Language:Chinese
Abstract: Objective:To establish an HPLC method to determine of vanillin in lysine and vitamin B12 granules.Methods:1.0 g of lysine and vitamin B12 granules was put into a 10mL graduated centrifuge tube,1 mL of water was added to dissolve,vortex for 1 min,and methanol was added to the scale and vortex for 1 min for extraction.The samples were separated on a C18 column(250 mm × 4.6 mm,5 μm)with a mobile phase of methanol and water(35∶65)at ta flow rate of 1.0 mL·min-1.The detection wavelength was 277 nm.Results:The limit of detection of vanillin was 0.2 μg·g-1,and the concentration was linear in the range of 1.962-49.04 μg·mL-1(r=0.999 9).The recoveries of vanillin at low,medium and high levels were 91.86％-101.61％with RSD of 2.9％(n=9).6 batches of samples were determined,and the results showed that the vanillin content in 4 batches of enterprise A was 55.18-79.55 μg·g-1,and no vanillin was detected in enterprise B and enterprise C.Conclusion:The method is simple and reliable,and can be used for the rapid determination of vanillin in lysine and vitamin B12 granules.