Norepinephrine promotes proliferation and migration of lung cancer A549 cells through CAFs
- VernacularTitle:去甲肾上腺素通过肿瘤相关成纤维细胞促进肺癌A549细胞增殖迁移
- Author:
Xi CHEN
1
;
Tao TANG
;
Peng-Yi YU
;
Hui WANG
Author Information
- Keywords: lung cancer; cancer-associated fibro-blasts; norepinephrine; proliferation; migration; tumor microenvironment
- From: Chinese Pharmacological Bulletin 2024;40(11):2093-2098
- CountryChina
- Language:Chinese
- Abstract: Aim To study the role of norepinephrine in regulating cancer-associated fibroblasts to promote the proliferation and migration of lung cancer cells.Meth-ods Primary cancer-associated fibroblasts and lung cancer A549 cells were cultured.RT-PCR and West-ern blotting were used to detect the expression of VEGF,IL-6 and IL-8 in cancer-associated fibroblasts after norepinephrine intervention,and the effect of nor-epinephrine on the expression of E-cadherin and vim-entin in co-cultured CAFs and lung cancer A549 cells were evaluated,and scratching experiments were used to verify that norepinephrine promoted the migration of lung cancer A549 cells.Results Norepinephrine in-duced the high expression of VEGF,IL-8 and IL-6 in CAFs cells in a concentration-dependent manner.β-adrenergic inhibitor(propranol)could inhibit the ex-pression of VEGF,IL-8 and IL-6 in CAFs cells.Norep-inephrine could inhibit the expression of E-cadherin in lung cancer A549 cells co-cultured with CAFs,promote the upregulation of vimentin,and enhance the migration ability of A549 cells.Conclusion Norepinephrine promotes the proliferation and migration of lung cancer A549 cells through CAFs.
