Antimicrobial activity of a novel R-type phage tail-like bacteriocin against MRSA
- VernacularTitle:新型R型噬菌体尾样细菌素对MRSA的抗菌活性研究
- Author:
Wei TANG
1
;
Ying LIU
;
Zhen-Hai TANG
;
Ying TANG
;
Xin LI
;
Jie YAO
;
Wei-Zu LI
;
Yuan-Hong XU
;
Qiang ZHOU
Author Information
- Keywords: Enterobacter cloacae; bacteriocin; phage tail-like bacteriocin; Staphylococcus aureus; methicil-lin-resistant Staphylococcus aureus; antimicrobial activ-ity
- From: Chinese Pharmacological Bulletin 2024;40(9):1760-1765
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the antimicrobial activity of a novel R-type phage tail-like bacteriocin(PTLB)secreted by Enterobacter cloacae SHAMU191747 a-gainst methicillin-resistant Staphylococcus aureus(MR-SA).Methods Antagonistic activity of E.cloacae SHAMU191747 against MRS A was detected by LB agar plate antagonistic test and LB broth micro-fermentation test.The crude extract of fermentation supernatant of E.cloacae SHAMU191747 was prepared by ultra-high-speed centrifugation and density gradient centrifuga-tion.The novel R-type PTLB in the crude extract was detected by transmission electron microscopy.The an-timicrobial activity of the crude extract against MRSA was verified by LB agar plate spot-seeding method.The molecular weight of the novel R-type PTLB was detected by SDS-PAGE electrophoresis.Results E.cloacae SHAMU191747 secreted a novel R-type PTLB,and had a strong antagonistic effect on MRSA.The no-vel R-type PTLB had a molecular weight of approxi-mately 35 ku and could efficiently kill MRSA.The physical dimensions of its tail-sheath-uncontracted functional molecules were(142.7±4.3)×(13.8±0.6)nm,and those of the tail-sheath-contracted non-functional molecules were(57.7±1.2)×(20.8±1.5)nm.Conclusions The novel R-type PTLB pro-duced by E.cloacae SHAMU191747 can efficiently kill MRSA,and has the potential to be developed into a novel antimicrobial drug with great prospects for clini-cal application.
