Expression pattern and function of novel gene C12ORF56 in lung cancer
- VernacularTitle:新基因C12ORF56在肺癌中的表达特征和功能研究
- Author:
Zeng ZHANG
1
;
Hua-Zhen XU
;
Run-He QIN
;
Yun-Min HUANG
;
Ya-Hao LING
;
Wen-Lin CHANG
Author Information
- Keywords: C12ORF56 gene; lung cancer; squamous cell lung carcinoma; cell proliferation; gene function prediction; expression pattern
- From: Chinese Pharmacological Bulletin 2024;40(9):1680-1687
- CountryChina
- Language:Chinese
- Abstract: Aim To disclose the subcellular localiza-tion,expression pattern,cellular physiological function and possible molecular mechanism of C12ORF56,a novel gene located at q14.2 of chromosome 12,in the pathogenesis of lung cancer.Methods ONCOMINE database was applied to investigate the mRNA level dif-fering of C12ORF56 between normal and lung cancer tissues.Analysis based on LinkedOmics,Metascape,String and GSEA database or tools provided indication of potential cellular physiological functions of C12ORF56 in the developing of lung cancer.C12ORF56 was knocked down via siRNA and the pro-liferation of NCI-H1073 cells were observed by EdU and CCK-8 assay.RT-qPCR was used to detect the ex-pression level of C12ORF56 of lung cancer cells on dif-ferent cycle phases.The core sequence regions of pro-moter affecting the transcription of C12ORF56 gene were analyzed by Jaspar online-tools and verified by dual-luciferase assay.Results C12ORF56 was highly expressed in lung cancer cells,especially in squamous cell lung cancer.C12ORF56 correlated with cell cy-cle,cancer immune,DNA replication.Knockdown of C12ORF56 reduced NCI-H1703 cell proliferation.Conclusion The up-regulation of C12ORF56 is in-volved in the development of lung cancer by enhancing lung cancer cell proliferation.
