Effect of Pien Tze Huang on emotional stress-induced influenza virus susceptibility
- VernacularTitle:片仔癀对情志内热诱导流感病毒易感性的作用研究
- Author:
Rong WANG
1
;
Xin-Xing CHEN
;
Rui-Ting HUANG
;
Wan-Yang SUN
;
Rong-Rong HE
;
Yi-Fang LI
;
Feng HUANG
Author Information
- Keywords: emotional stress; Pien Tze Huang; heat-clearing Chinese medicine; influenza viral pneumonia; oxidative stress; phospholipid peroxidation
- From: Chinese Pharmacological Bulletin 2024;40(8):1565-1572
- CountryChina
- Language:Chinese
- Abstract: Aim To evaluate the effect of Pien Tze Huang(PTH)on influenza virus susceptibility in re-straint stress-induced H1N1 influenza susceptibility model in mice with emotional disorders and internal heat,guided by the theory of emotional pathogenesis.Methods Mice were infected with H1N1 influenza vi-rus following 18 h of restraint stress.The signs and weight changes of mice were recorded,and the morbid-ity of mice were analyzed.On the fourth day post viral infection,the lung tissue was collected.The pathologi-cal changes and inflammatory factors in lungs were de-tected by HE staining.The expression of NP was as-sessed by immunohistochemistry and Western blot.The level of lipid peroxidation end products was detected u-sing a commercial kit and western blot.Redox phos-pholipidomics was analyzed in lung tissue by HPLC-MS/MS.Results PTH significantly reduced the mor-tality of influenza-susceptible mice induced by emotion-al stress,inhibited the expression of NP and the re-lease of inflammatory factors,improved inflammation in lung tissue,and alleviated the accumulation of lipid peroxidation end products.Phospholipid oxidation a-nalysis revealed the elevated levels of oxidized phos-pholipid choline and phosphatidylethanolamine in lung tissue of influenza-susceptible mice,which were signif-icantly reduced by PTH administration.Conclusions PTH exhibits promising efficacy in ameliorating influ-enza virus susceptibility induced by internal heat,and its mechanism of action may be related to the regulation of phospholipid peroxidation.
