Effect of CXCL7/CXCR2 axis on synaptic plasticity in obesity-related cognitive dysfunction
- VernacularTitle:CXCL7/CXCR2轴调控突触可塑性在肥胖相关认知功能障碍中的作用
- Author:
Jia HU
1
;
Ao-Xue XU
;
Rui HU
;
Qi XUE
;
Chun-Xia HUANG
;
Ye ZHANG
Author Information
- Keywords: CXCR2; CXCL7; obesity; cognition dis-function; neuroinflammation
- From: Chinese Pharmacological Bulletin 2024;40(5):881-886
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the effect of CXCL7/CX-CR2 axis on obesity-related cognitive dysfunction at both animal and cellular levels.Methods The novel object recognition test was performed to assess the cog-nition.After the preparation of the frozen sections,the activation of microglia and astrocytes in hippocampi and the level of PSD95 were determined by immunoflu-orescence staining.The content of CXCL7 in hipp-ocampi was determined by enzymelinked immunosor-bent assay.The dendritic spine density of hippocampal neurons was observed by Golgi staining.Furthermore,HT22 cells were treated with the recombinant mouse CXCL7 and/or si-RNA targeting CXCR2.After the treatment,the levels of CXCL7 and PSD95 were ob-served by immunocytochemistry staining.Results Compared with animals in the control group,there was significantly decreased discrimination index,increased activation of microglia and astrocytes,decreased con-tent of PSD95,decreased density of dendritic spine,and increased content of CXCL7 in hippocampi in the DIO group.Compared with animals in the DIO group,there were significantly increased discrimination index in the AWL group.In HT22 cells,the level of PSD95 significantly decreased in the Ctrl+CXCL7 group com-pared with the control group.This decrease was attenu-ated in the si-CXCR2+CXCL7 group compared with the Ctrl+CXCL7 group.Conclusion Chronic high-fat diet induces neuroinflammation and subsequently induces cognitive dysfunction,which may be related to the synaptic plasticity mediated by the CXCL7/CXCR2 axis.
