Improved cognitive function and brain aging in SAMP8 mice after Aβ3-10KLH vaccine
10.12007/j.issn.0258-4646.2024.11.004
- VernacularTitle:Aβ3-10KLH疫苗改善SAMP8小鼠认知功能和脑衰老
- Author:
Xin LIU
1
;
Yunpeng CAO
;
Chuansheng ZHAO
Author Information
1. 中国医科大学附属第一医院神经内科,沈阳 110001;北华大学附属医院神经二科,吉林吉林 132011
- Keywords:
Alzheimer disease;
brain aging;
oxidative stress
- From:
Journal of China Medical University
2024;53(11):979-983
- CountryChina
- Language:Chinese
-
Abstract:
Objective This study aimed to investigate the effects of Aβ3-10KLH active immunization on brain aging in the SAMP8 rapid aging mouse model using oxidative stress as the entry point.Methods SAMP8 mice were immunized with the Aβ3-10KLH vaccine,and a control group was established.Behavioral changes were observed using the Morris water maze test,and hippocampal morphological changes were examined using hematoxylin and eosin staining.The proportion of senescent cells in the hippocampus was detected using SA-β-gal staining.Biochemical kits were used to measure the changes in oxidative stress injury indicators malondialdehyde(MDA)and superoxide dismutase(SOD)activities to assess oxidative stress damage in the hippocampus.Results The Aβ3-10KLH vaccine attenua-ted cellular aging in the hippocampi of SAMP8 mice.It also improved memory ability(P＜0.05),ameliorated hippocampal tissue mor-phology,decreased the proportion of senescent cells in the hippocampus(P＜0.05),lowered MDA content(P＜0.05),and upregulated SOD activity(P＜0.05).Conclusion The Aβ3-10KLH vaccine significantly improves cognitive function in SAMP8 mice,slows down the aging of hippocampal neurons,and reduces oxidative stress damage.
