TXN expression in pancreatic cancer and its clinical signifi-cance
10.3969/j.issn.1009-9905.2024.05.004
- VernacularTitle:硫氧还蛋白在胰腺癌中的表达及临床意义
- Author:
Lin-Hai XU
1
;
Mei-Na LI
;
Xiao HU
Author Information
1. 青岛大学医学部(山东 青岛 266000);青岛大学附属医院 肝胆胰外科(山东 青岛 266000);青岛市胶州中心医院 肝胆外科(山东 青岛 266300)
- Keywords:
Thioredoxin;
Pancreatic cancer;
Proliferation;
Invasion;
Migration
- From:
Chinese Journal of Current Advances in General Surgery
2024;27(5):353-358
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Investigate the expression of thioredoxin(TXN)in pancreatic cancer and its impact on the proliferation,invasion,migration,and apoptosis of pancreatic cancer cells.Methods:By collecting pan-cancer and TCGA pancreatic cancer transcriptome and clinical data,the expression levels of the TXN gene family were analyzed.The expression levels of TXN in cancer tissues,adjacent tissues,and different cell lines were detected by fluorescence quantitative PCR(RT-qPCR)and Western blotting.The expression of the TXN gene in Panc-1 and BxPC-3 pancre-atic cancer cells was inhibited using small interfering RNA(siRNA),and control experiments were conducted with Panc-1 and BxPC-3 cells with unblocked TXN gene expression to explore the ef-fects of TXN on the proliferation,migration,invasion,and apoptosis of these two types of cells.Transcriptional data and survival outcome data were used to divide TXN expression into two groups based on the median value,and survival curves of the high and low TXN expression groups were plotted.Results:In pancreatic cancer tissues and cells,the expression level of TXN was signifi-cantly higher than that in adjacent tissues and normal cells(P＜0.05).After inhibiting the expression of TXN in pancreatic cancer cells,the proliferation rate,invasion cell number,and healing rate of pancreatic cancer cells with low TXN expression were lower than those of the control group,and their apoptosis rate was significantly higher than that of normal pancreatic cancer cells(P＜0.05).The higher the expression level of TXN in the tumors of pancreatic cancer patients,the shorter their survival time(P＜0.05).Conclusion:TXN can enhance the proliferation,migration,and invasion ability of pancreatic cancer cells and weaken the degree of cell apoptosis;the higher the expression level of TXN in pancreatic cancer tissues,the worse the prognosis.
