Study on the Production of Anti-D and Anti-E Mixed Antibodies by Alloimmunization in RhD Variant Type33 Recipients

VernacularTitle:RhD变异体Type33型受血者同种免疫产生抗-D合并抗-E混合抗体的研究
Author: Jian-Cheng LIU ¹ ; Feng SHAO
Author Information

1. 宁夏血液中心血型参比实验室,宁夏银川750000
Keywords: weak D type33; RhD variant; RhD blood group; RHD gene; mixed antibody; blood receivers
From: Journal of Experimental Hematology 2024;32(6):1859-1864
CountryChina
Language:Chinese
Abstract: Objective:To investigate the cause of the production of anti-D and anti-E mixed antibody in an RhD positive patient.Methods:The ABO/Rh blood group typing and irregular antibody specificity were identified by conventional serological methods,the RHD gene exon 1-10 and heterozygous analysis were performed by sequence-specific primer polymerase chain reaction (PCR-SSP),and the whole exon sequence was analyzed by first-generation sequencing.Results:The patient's Rh blood group was weak D Type33,with the allele was RHD*01W.33,the patients was found to be RhD+/RHD-heterozygous,with an Rh typing of Ccee,and the patient had developed anti-D combined with anti-E mixed antibodies.Conclusion:The patient has A c.520G>A mutation in exon 4 of the RHD gene,to lead decreased expression of RhD antigen in red blood cells and the anti-D and anti-E mixed antibodies were produced by transfusion immunostimulation.