Inhibition Effect of Resveratrol on Spontaneous Senescence of Human Bone Marrow-Derived Mesenchymal Stem Cells
10.19746/j.cnki.issn1009-2137.2024.04.039
- VernacularTitle:白藜芦醇对人骨髓间充质干细胞自发衰老的抑制作用
- Author:
Yue YANG
1
;
Jian-Xin HE
Author Information
1. 西北民族大学医学部
- Keywords:
human bone marrow-derived mesenchymal stem cell;
resveratrol;
spontaneous senescence;
hTERT;
mTOR
- From:
Journal of Experimental Hematology
2024;32(4):1224-1229
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore whether Resveratrol(RSV)can inhibit the spontaneous senescence of human bone marrow-derived mesenchymal stem cells(MSC).Methods:MSC were serially cultured to passage 13 and passage 15 to establish model groups exhibiting spontaneous senescence,respectively.MSC at passage 13 and passage 15 were treated with 5 nmol/L RSV for 48 h to establish the RSV-treated groups.SA-β-Gal staining was used to detect cell senescence.MTT assay was used to detect cell proliferation.RT-PCR method was used to detect senescence-associated telomerase activity.Western blot was used to detect the senescence-associated protein level of the phosphorylated-mTOR.Results:SA-β-Gal staining showed that the senescent cells of MSC in RSV-treated group was significantly less than those in the model group(RSV group compared with model group at passage 13,P＜0.05;RSV group compared with model group at passage 15,P＜0.01).The cell proliferation ability of MSC in RSV-treated group was significantly higher than those in model group,at 72 h in passage 13,there was significant difference between RSV-treated group and model group(P＜0.05).RT-PCR results showed that the hTERT mRNA expression of MSC in RSV-treated group was higher than that in model group,which was significantly different between RSV-treated group and model group at passage 13(P＜0.05).Western blot results showed that the phosphorylated(Ser2448)-mTOR level of MSC in RSV-treated group was lower than that in model group,which was significantly different between RSV-treated group and model group at passage 13(P＜0.05).Conclusion:RSV can inhibit the spontaneous senescence of human MSC by mediating mTOR activity.
