Clinical Significance of the Levels of Peripheral Blood Tregs and Cytokines IL-35,TGF-β and IL-10 in Hemophilia A Patients with FⅧ Inhibitor
10.19746/j.cnki.issn1009-2137.2024.04.034
- VernacularTitle:外周血调节性T细胞及细胞因子IL-35、TGF-β和IL-10水平在血友病A抑制物阳性患者中的临床意义
- Author:
Hong-Xia HE
1
;
Yan-Yan XIE
;
Qing-Yun SUN
;
Lin-Hong WANG
;
Yi-Wen ZHU
;
JIE LI
;
Xin WANG
;
Zhao-Ling DENG
;
Mei-Rong YANG
;
Zhen-Yu YAN
Author Information
1. 华北理工大学附属医院血液科,河北唐山 063000
- Keywords:
hemophilia A;
FⅧ inhibitor;
regulatory T cell;
cytokines
- From:
Journal of Experimental Hematology
2024;32(4):1197-1200
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the levels of regulatory T cells(Tregs)and cytokines IL-35,TGF-β and IL-10 in peripheral blood of hemophilia A(HA)patients with F Ⅷ inhibitor and their clinical significance.Methods:43 HA patients admitted to the Hematology Department of the Affiliated Hospital of North China University of Science and Technology from October 2019 to December 2020 were selected,including 6 cases with F Ⅷ inhibitor and 37 cases without FⅧ inhibitor.In addition,20 healthy males who underwent physical examinations were selected as healthy controls.Flow cytometry was used to detect the levels of CD4+CD25+CD127-Tregs in peripheral blood of the HA patients and healthy controls,and ELISA assay was used to detect the expression levels of IL-35,TGF-β and IL-10 in serum,and their differences between different groups were compared.Results:Compared with the healthy control group,the level of Tregs in HA patients was decreased,and the level of Tregs in the FⅧ inhibitor positive group was the lowest,the difference was statistically significant(P＜0.05).There was no significant difference in the expression level of Tregs in HA patients of different severity levels.The serum IL-35,TGF-β,and IL-10 levels in both FⅧ inhibitor negative and positive groups were significantly lower than those in healthy control group,and those in FⅧ inhibitor positive group were significantly lower than those in FⅧ inhibitor negative group(all P＜0.05).Conclusion:The decrease of Tregs,IL-35,TGF-β,and IL-10 levels in HA patients may be related to the formation of FⅧ inhibitors.
