Thiotert Induces Myelodysplastic Syndromes Cells Apoptosis by Activating Oxidative Stress
10.19746/j.cnki.issn1009-2137.2024.04.031
- VernacularTitle:6-异丙基双硫-2'-脱氧鸟苷通过氧化应激诱导骨髓增生异常综合征细胞凋亡
- Author:
Qiang-An JING
1
;
Chao-Ting ZHOU
;
Yun-Yi WU
;
Xia KE
;
Xiang-Min TONG
Author Information
1. 浙江省人民医院(杭州医学院附属人民医院)临床医学研究所,浙江杭州 310006;浙江工业大学生物工程学院,浙江杭州 310014
- Keywords:
myelodysplastic syndromes;
thiotert;
reactive oxygen species;
apoptosis
- From:
Journal of Experimental Hematology
2024;32(4):1181-1185
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore whether thiotert treatment can inhibit proliferation and induce apoptosis in myelodysplastic syndromes(MDS)cells.Methods:CCK-8 assay was used for determining the cytotoxicity of thiotert to MDS cell line SKM-1 and the reversal effect of GSH,NAC,and Z-VAD-FMK on thiotert-induced inhibition of cell viability.EdU assay was deployed to detect the cell proliferation ability.Intracellular reactive oxygen species(ROS)was measured by flow cytometry after DCFH-DA staining.The expression of DNA damage-and apoptosis-related proteins was detected by Western blot.Results:Thiotert treatment significantly suppressed the cell viability and proliferation ability in SKM-1 cells.A large amount of ROS generation and markedly elevated C-PARP,C-Caspase 3,and γ-H2AX were observed after thiotert administration,while BCL-2 was significantly decreased.In addition,GSH,NAC,and Z-VAD-FMK were able to mitigate the cytotoxicity of thiotert on SKM-1 cells.Conclusion:Thiotert can promote MDS cell apoptosis by mediating ROS production and pro-apoptotic proteins expression.
