Quantitative Detection and Integrality Analysis of Plasma Circulating Cell-Free DNA in Multiple Myeloma
10.19746/j.cnki.issn1009-2137.2024.04.020
- VernacularTitle:多发性骨髓瘤血浆循环游离DNA定量检测和完整性分析
- Author:
Lan-Xin ZHANG
1
;
Yi-Zhi JIANG
;
Li-Jun QIU
;
Dong-Ping HUANG
Author Information
1. 皖南医学院第一附属医院弋矶山医院血液科,安徽芜湖 241000
- Keywords:
newly diagnosed multiple myeloma;
circulating free DNA;
ALU247;
ALU115;
integrality
- From:
Journal of Experimental Hematology
2024;32(4):1106-1111
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the role of plasma circulating cell-free DNA(cf-DNA)in the screening and diagnosis of patients with newly diagnosed multiple myeloma(MM)and explore the changes of cf-DNA in terms of content and integrality in the assessment of disease in patients treated with chemotherapy.Methods:Peripheral blood specimens were collected from 35 newly diagnosed MM patients and 18 healthy volunteers,and 13 of the 35 patients who had finished 3 courses of standard induction chemotherapy were selected as follow-up group.The ALU247 and ALU115 fragments were used as the target genes,and the cf-DNA content in the plasma of patients and healthy controls was measured by quantitative polymerase chain reaction(qPCR).The integrality of cf-DNA was calculated by the ratio of ALU247 to ALU115.Results:Both the concentration of ALU247 and ALU115 fragments and the integrality of cf-DNA in patients were significantly higher than those in healthy controls(all P＜0.05).Patients who had underwent 3 courses of induction chemotherapy had significantly decreased concentration of ALU247 and ALU115 fragments and integrality of cf-DNA after treatment(all P＜0.05),and strong positive correlations were manifested between cf-DNA integrality and serum M protein content,as well as proportion of abnormal plasma cells in bone marrow before and after treatment(r=0.703,0.705).Conclusions:Cf-DNA has certain positive significance for the screening and diagnosis of MM.Furthermore,cf-DNA may be a synergism or alternative to serum M-protein content and proportion of abnormal plasma cells in bone marrow in assessing the condition,curative effect and prognosis of patients.
