Resveratrol Inhibits T-acute Lymphoblastic Leukemia in Mice by Regulating Notch1 Signaling Pathway
10.19746/j.cnki.issn1009-2137.2024.01.010
- VernacularTitle:白藜芦醇抑制Notch1信号通路对抗小鼠急性T淋巴细胞白血病
- Author:
Xiao-Fei LI
1
;
Fang CUI
;
Fei LIU
;
Rui ZHANG
;
Min SHI
;
Yong-Jun LI
Author Information
1. 河北医科大学第二医院检验科,河北石家庄 050000
- Keywords:
resveratrol;
T-acute lymphoblastic leukemia;
Notch1 signaling pathway;
mice
- From:
Journal of Experimental Hematology
2024;32(1):57-65
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of resveratrol(Res)on T-acute lymphoblastic leukemia(T-ALL)mice,and further explore its mechanism on Notch1 signaling pathway.Methods:Twenty-five 6-8 weeks old female C57BL/6 mice were randomly divided into control group,T-ALL group and Res group.Res group was further divided into low-Res.middle-Res and high-Res group.The percentage of leukemia cells in peripheral blood and spleen cell suspension were detected by flow cytometry and Wright-Giemsa staining,pathological morphology of spleen and bone marrow tissues were observed by HE staining,the expression levels of Notch1,Hes-1,c-Myc,miR-19b and PTEN mRNA in spleen tissue were detected by RT-qPCR,and the protein levels of Notch1,Hes-1,c-Myc,p-PTEN and PTEN were detected by Western blot.Results:Compared with control group,the leukemia cells in peripheral blood of mice in T-ALL group were markedly increased,accompanied by diffuse infiltration of leukemia cells in spleen and bone marrow tissues,the mRNA levels of Notch1,Hes-1,c-Myc,miR-19b and the protein levels of Notch1.Hes-1,c-Myc were increased(P＜0.01),while the expression of PTEN mRNA and protein were significantly decreased in the spleen tissue of T-ALL mice(P＜0.01).The above indicators in the H-Res group were reversed compared with T-ALL group after administration of resveratrol.Conclusion:Resveratrol may play a role in anti T-ALL by inhibiting Notch1 signaling pathway in mice.
