Mechanism of HMGCR on liver bile acid and lipid metabolism in dairy cow with fatty liver
10.16303/j.cnki.1005-4545.2024.11.19
- VernacularTitle:HMGCR对脂肪肝奶牛肝脏胆汁酸及脂质代谢机制
- Author:
Changhong GAO
1
;
Shuang WANG
;
Yan TIAN
;
Wenwen FAN
;
Jie LI
;
Wei YANG
Author Information
1. 黑龙江八一农垦大学动物科技学院,黑龙江大庆 163319
- Keywords:
dairy cow;
fatty liver;
bile acids;
cholesterol;
HMGCR
- From:
Chinese Journal of Veterinary Science
2024;44(11):2452-2457
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the mechanism of 3-hydroxy-3-methylglutaryl-CoA reductase(HMGCR)on liver bile acid(BAs)and lipid metabolism of dairy cows with fatty liver,A liver lip-id accumulation model was established by isolating primary calf hepatocytes and treating them with high concentration of non-esterified fatty acids(NEFA)in vitro.Then,HMGCR overex-pressed adenovirus(Ad-HMGCR)and overexpressed adenovirus control(Ad-GFP)were added.Hepatocyte triglyceride(TAG)was detected by the kit,lipid droplet changes were detected by lip-id droplet fluorescence,and BAs synthesis,fatty acid synthesis and oxidation factor changes were detected by real-time fluorescence quantitative PCR.The results showed that TAG content and lip-id droplet fluorescence were significantly reduced in Ad-HMGCR+NEFA group compared with Ad-GFP+NEFA group.Real-time fluorescence quantitative PCR results showed that CYP7A1,CYP8B1,CYP7B1 and CYP27A1 of hepatocyte BAs synthesis factors in Ad-HMGCR+NEFA group,BAs transporters ABCC2 and ABCB11 and fatty acid synthesis factors ACC1,FAS and SREBP1C were significantly lower than those in Ad-GFP+NEFA group.The levels of BAs syn-thesis factor FXR and lipid oxidation factor CPT1A in Ad-HMGCR+NEFA group were higher than those in Ad-GFP+NEFA group.The results showed that overexpression of HMGCR could significantly reduce BAs and lipid accumulation in the liver of dairy cows with fatty liver.
