Preparation and immunological performance identification of ribavirin mono-clonal antibodies
10.16303/j.cnki.1005-4545.2024.09.23
- VernacularTitle:利巴韦林单克隆抗体的制备及免疫学性能鉴定
- Author:
Xiaofei HU
1
;
Fengxian WEI
;
Yunrui XING
;
Lin WANG
;
Yaning SUN
Author Information
1. 河南省农业科学院动物免疫学重点实验室,河南郑州 450002
- Keywords:
ribavirin;
glutaraldehyde method;
artificial complete antigen;
cell fusion;
monoclonal an-tibody
- From:
Chinese Journal of Veterinary Science
2024;44(9):2025-2030
- CountryChina
- Language:Chinese
-
Abstract:
This study aims to obtain highly sensitive and specific monoclonal antibodies to ribavirin.Ribavirin was coupled with carrier proteins to synthesize artificial complete antigen by glutaralde-hyde method.UV scanning,gel electrophoresis and animal immunization were employed to identify quality of the synthesized artificial complete antigens.The monoclonal antibody was prepared by cell fusion,positive hybridoma screening and induction of ascites in vivo,and the immunological characteristics of monoclonal antibodies were identified.The results showed that RBV was coupled to carrier proteins,and the artificial antigens were prepared successfully.One cell line that can stably secrete monoclonal antibodies against ribavirin was obtained,and the titers of the mono-clonal antibodies secreted by cell line was above 1∶512 000,with IC50 values of 4.74 μg/L.Fur-thermore,there were no cross-reactivity with other antiviral drugs such as amantadine and carrier proteins.In this study,monoclonal antibodies of ribavirin with high sensitivity and specificity was synthesized successfully,which lays a solid foundation for the establishment of immunoassay methods of ribavirin.
