Exploring therapeutic effects of Forsythia suspensa extracts on rat mastitis based on Toll-like signaling pathway
10.16303/j.cnki.1005-4545.2024.07.23
- VernacularTitle:基于Toll样信号通路探讨FSE对大鼠乳腺炎的治疗作用
- Author:
Xiaoli CHENG
1
;
Lu CAO
;
Tao WANG
;
Dan YAO
;
Yong ZHANG
Author Information
1. 甘肃农业大学动物医学院,甘肃兰州 730070;甘肃省动物生殖生理及繁殖调控重点试验室,甘肃兰州 730070
- Keywords:
Forsythia suspensa;
Staphylococcus aureus;
mastitis;
TLR4;
MyD88;
rat
- From:
Chinese Journal of Veterinary Science
2024;44(7):1521-1525,1570
- CountryChina
- Language:Chinese
-
Abstract:
The aim of the present study was to investigate the protective mechanism of Forsythia suspensa extracts(FSE)on S.aureus-induced inflammatory injury of rat mammary epithelial cells.Fifty lactating rats were selected and randomly divided into five groups with 10 rats each:the blank control group(the fourth pair of mammary glands were injected with saline),the model group(the fourth pair of mammary glands were injected with 100 pL of S.aureus bacterial solution),the high-dose group(the fourth pair of mammary glands were injected with S.aureus bacterial solu-tion for 24 h,and then the rats were administered by gavage of 3.750 g/(kg·d)FSE for 1-7 d),the medium-dose group(24 h after the fourth pair of mammary injections,the rats were adminis-tered by gavage 1.875 g/(kg·d)FSE for 1-7 d),and in the low-dose group(24 h after the fourth pair of mammary injections,the rats were administered by gavage 0.935 g/(kg·d)FSE for 1-7 d),blood samples were collected 24 h after the last administration of the drug and then the rats were executed.The results showed that the mammary tissue of the model group was severely dam-aged,and the mRNA expression of IKKβ,MyD88,NF-κB,and TLR4 was significantly increased in the mammary tissue(P＜0.05).The relative expression of IKKβ,MyD88,NF-κB,and TLR4 pro-teins in the mammary tissue of the model group was significantly decreased(P＜0.05)by medium and high doses of FSE compared with the model group.Therefore,FSE may play an anti-inflammatory role by inhibiting the Toll-like signaling pathway to improve S.aureus-induced inflammatory re-sponse in rat mammary epithelial cells and decrease the expression of the downstream pathway proteins IKKβ,MyD88,NF-κB,and TLR4.
