MiR-513b-5p Inhibits Proliferation,Migration and Invasion of Laryngeal Squamous Cell Carcinoma by Targeting CXCL8
10.13865/j.cnki.cjbmb.2024.09.1186
- VernacularTitle:miR-513b-5p靶向趋化因子CXCL8抑制喉鳞癌细胞增殖、迁移和侵袭
- Author:
Yu-Liang ZHANG
1
;
Xu-Ting XUE
;
Ying WANG
;
Long HE
;
Xiao-Ya GUAN
;
Chun-Ming ZHANG
Author Information
1. 山西医科大学第一医院,耳鼻咽喉头颈肿瘤山西省重点实验室,太原030001
- Keywords:
laryngeal squamous cell carcinoma (LSCC);
miR-513b-5p;
CXCL8;
proliferation;
invasion
- From:
Chinese Journal of Biochemistry and Molecular Biology
2024;40(11):1627-1635
- CountryChina
- Language:Chinese
-
Abstract:
Metastasis is the main risk factor for poor prognosis of laryngeal squamous cell carcinoma (LSCC) .Chemokines are closely related to metastasis in the tumor microenvironment.CXCL8 is a cyto-kine-like secreted protein that plays key roles in the malignant development of a variety of tumors,but has not been elucidated in LSCC.In this paper,we elucidated the role of CXCL8 in LSCC cells and found miRNAs that targeted CXCL8,which may become new targets for the diagnosis and treatment of LSCC.Firstly,the GEPIA showed that CXCL8 was highly expressed in head and neck cancer (P<0.05).The real-time fluorescence quantification (qRT-PCR) found that CXCL8 was highly expressed in LSCC cells.The enzyme-linked immunoassay also found that CXCL8 was highly secreted in the superna-tant of LSCC cells (P<0.001) .Then,the CCK8 assay confirmed that knockdown of CXCL8 significant-ly inhibited the proliferation of FD-LSC-1 and AMC-HN8 cells (the average inhibition rate was 34.0％and 19.5％,respectively);The EdU assay also confirmed that knockdown of CXCL8 significantly inhibi-ted the proliferation of LSCC cells (P<0.05) .The transwell assay confirmed that knockdown of CXCL8 also significantly inhibited the migration and invasion of FD-LSC-1 cell (average inhibition rate was 40.0％,38.5％,respectively);Knockdown of CXCL8 also significantly inhibited the migration and inva-sion of AMC-HN8 cell (average inhibition rate was 37.5％,53.5％,respectively ) .The analysis of bioinformatics predicted that CXCL8 may be a target of miR-513b-5p.The dual luciferase reporter assay confirmed that miR-513b-5p could bind to the CXCL8-3'UTR.QRT-PCR assay also confirmed that over-expression of miR-513b-5p could decrease the 60％ of the CXCL8 expression (P<0.01) .Cell function rescue assays found that overexpressed of CXCL8 could effectively reversed proliferation,migration and invasion of LSCC cells weakened by miR-513b-5p (P<0.05) .In summary,miR-513b-5p inhibited the proliferation,migration and invasion of LSCC cells by targeting CXCL8.
