C/EBPα Positively Regulates Chicken NRG4 Gene Expression via Binding to Its Exon 2
10.13865/j.cnki.cjbmb.2024.09.1192
- VernacularTitle:C/EBPα通过结合鸡神经调节蛋白4基因外显子2正调控其表达
- Author:
Hao-Yu LUO
1
;
Jia-Xin HUANG
;
Fan GAO
;
Xiao-Hong YAN
;
Fang MU
;
Ning WANG
Author Information
1. 农业农村部鸡遗传育种重点实验室,哈尔滨150030;黑龙江省普通高等学校动物遗传育种与繁殖重点实验室,哈尔滨150030;东北农业大学动物科学技术学院动物遗传育种与繁殖系,哈尔滨150030
- Keywords:
neuregulin 4 (NRG4);
proximal exon and intron;
promoter activity;
transcriptional regulation;
CCAAT/enhancer-binding protein alpha (C/EBPα)
- From:
Chinese Journal of Biochemistry and Molecular Biology
2024;40(11):1608-1617
- CountryChina
- Language:Chinese
-
Abstract:
Neuregulin 4 (NRG4) is a newly discovered adipokine,which plays crucial roles in many physiological processes such as energy balance,and glucose and lipid metabolism.The transcriptional regulation of NRG4 gene remains largely unknown.Our previous 5'RACE analysis showed that the tran-scription start sites (TSS) of chicken NRG4 gene were dispersed over a region of 200 bp,suggesting that chicken NRG4 gene possesses a dispersed promoter.In the present study,the promoter activity of the proximal exon and intron of chicken NRG4 gene was analyzed.Dual-luciferase reporter assay demonstra-ted that the genomic fragment (-122/+452) containing exon 1,intron 1,and exon 2 of chicken NRG4 gene had strong bidirectional promoter activity.Bioinformatics analysis showed that there was a putative binding site of the transcription factor CCAAT enhancer-binding proteinα(C/EBPα) in exon 2 of chick-en NRG4 gene.Reporter gene assay showed that both deletion and site-directed mutagenesis of the C/EBPα binding site abrogated the regulatory effect of C/EBPα on the promoter activity of the-122/+452 fragment of chicken NRG4 gene.Gene expression correlation analysis showed that C/EBPα and NRG4 gene expression were positively correlated in chicken adipose tissues.ChIP-PCR revealed that C/EBPα directly binds to the exon 2 of NRG4 gene in chicken adipose.In conclusion,the proximal exon and intron of chicken NRG4 gene has bidirectional promoter activity,and C/EBPα directly regulates NRG4 gene expression in chicken adipose via binding to its exon 2.
