Exosomal PD-L1 Detection Methods Based on Surface Plasmon Resonance
10.13865/j.cnki.cjbmb.2024.07.1136
- VernacularTitle:基于表面等离子体共振的外泌体PD-L1蛋白检测技术
- Author:
Li-Si QU
1
;
Yu-Yan PENG
;
Ze-Tao YU
;
Zi-Hong YE
;
Wen-Qiang XIA
Author Information
1. 中国计量大学生命科学学院,浙江省生物计量及检验检疫技术重点实验室,杭州 310018
- Keywords:
exosome;
programmed cell death 1 ligand 1(PD-L1);
α-hemolysin;
biomarker
- From:
Chinese Journal of Biochemistry and Molecular Biology
2024;40(9):1300-1307
- CountryChina
- Language:Chinese
-
Abstract:
Soluble programmed cell death 1 ligand 1(PD-L1)in the serum includes exosomal,mi-crovesical and secreted forms of PD-L1.Previous studies have shown that the level of exosomal PD-L1 in the serum significantly correlated with the prognosis of various cancers.However,current analysis detects all forms of PD-L1 in the serum as a whole,without distinguishing exosomal PD-L1 from other forms.In this study,a specific detection method for exosomal PD-L1 was established based on surface plasmon res-onance.This method first captures PD-L1 by antibody recognition and immobilizes it on the surface of the detection chip.Then,α-hemolysin was recruited to form multiple oligomers on the exosomal membrane.This method quantifies the content of exosomal PD-L1 by monitoring the signal change during the binding process of α-hemolysin,effectively reducing background noises and amplifying the signal.The linear range before signal amplification with α-hemolysin was 0.035-2.208 pg/mL,and after signal amplifica-tion,it was 0.004-0.552 pg/mL.Methodological validation showed that this method has good specifici-ty,sensitivity,and repeatability,and has certain clinical application prospects.
