Lipidomic Analysis Revealed the Regulatory Mechanism of High Altitude Hypoxia on Phospholipid Metabolism in Mouse Spleen Tissue
10.13865/j.cnki.cjbmb.2024.07.1132
- VernacularTitle:基于脂质组学分析揭示高原低氧对小鼠脾组织磷脂代谢的调控机制
- Author:
Xin WANG
1
;
Yu-Jing GUO
;
Jia-Yang WANG
;
Xiao-Jun WANG
;
Ying HU
Author Information
1. 青海大学医学院基础医学部免疫学教研室,西宁 810016
- Keywords:
hypoxia;
spleen;
lipidomics;
phosphatidylethanolamine(PE);
cardiolipin
- From:
Chinese Journal of Biochemistry and Molecular Biology
2024;40(9):1289-1299
- CountryChina
- Language:Chinese
-
Abstract:
The spleen is the largest lymphoid organ of the body,which participates in the regulation of metabolic balance.High altitude hypoxia environment can affect lipid metabolism in spleen tissue,but the key mechanism of lipid metabolism is still unclear.We aimed to use lipidomic analysis to study the effect of high altitude hypoxia on lipid metabolism in mouse spleen tissue.C57BL/6 mice were placed at an altitude of 4 200 m and 400 m,respectively,and after 30 days the spleen tissues were harvested and lipidomic analysis was performed using an ultra-high performance liquid chromatography-Orbitrap mass spectrometry system.Under the high altitude hypoxia environment,the spleen index of mice and the white pulp decreased,and the germinal center expanded with other pathological changes.The results of lipidomic analysis showed that a total of 41 lipid subclasses and 2 473 lipid molecules were identified,and triacylglycerides(TGs)and phosphatidylcholines(PCs)were the two most identified lipid mole-cules.Using univariate and multivariate analysis,44 differentially expressed lipid molecules were identi-fied,which were mainly concentrated in phospholipid metabolism.Subsequently,RT-qPCR was per-formed on the key enzymes in the phospholipid metabolic pathway,and it was found that the mRNA ex-pression levels were different(P＜0.05).It suggested that high altitude hypoxia environment mainly af-fects the phospholipid metabolism of mouse spleen tissue via reducing the contents of PC and phosphatidic acid(PA),promoting their conversion to phosphatidylethanolamine(PE)and cardiolipin(CL)and fa-cilitating the PE production via the CDP-Etn pathway.This study provides a new experimental basis for the abnormal metabolism of phospholipids in spleen tissue under high altitude hypoxia environment.
