A QCM Biosensor for Screening Arsenic(Ⅲ)Aptamers and Detecting Arsenic(Ⅲ)

VernacularTitle:一种用于筛选砷(Ⅲ)适配体和检测砷(Ⅲ)的QCM生物传感器
Author: Chu-Jun ZHENG ¹ ; Shi-Quan QIAN ; Xin-Pei LI ; Xu YAN ; Hai-Xuan HUANG ; Yu-Xuan WANG ; Yu-Wei YE ; Min YUAN
Author Information

1. 上海理工大学健康科学与工程学院,上海食品快速检测工程技术研究中心,上海 200093
Keywords: arsenic(Ⅲ); aptamer; quartz crystal microbalance(QCM)-systematic evolution of ligands by exponential enrichment(SELEX)(QCM-SELEX); sequencing
From: Chinese Journal of Biochemistry and Molecular Biology 2024;40(9):1282-1288
CountryChina
Language:Chinese
Abstract: A quartz crystal microbalance(QCM)-systematic evolution of ligands by the exponential en-richment(SELEX)technique was developed to screen out aptamers with high affinity for arsenic(Ⅲ).A random single strand DNA library was designed and fixed on the mercaptoethylamine-modified crystal plate with arsenic(Ⅲ)as the target,and the free aptamer was captured in the solution,and the QCM-SELEX screening method was constructed.After 6 rounds of screening,the secondary library was se-quenced with high throughput method,and the 6S1 dissociation coefficient Kd value was 0.36 μmol/L based on QCM resonance frequency.Using 6S1 as a probe,the QCM biosensor was constructed for the detection of arsenic(Ⅲ).The sensor has a good linear relationship in the range of 0.01 μmol/L～0.2μmol/L,and the detection limit of arsenic(Ⅲ)is 5.2 nmol/L(3σ),indicatinggood selectivity.