Long non-coding RNA PART1 Inhibits Proliferation and Invasion of Laryngeal Squamous Carcinoma Cells
10.13865/j.cnki.cjbmb.2024.05.1037
- VernacularTitle:长非编码RNA PART1抑制喉鳞癌细胞增殖和侵袭
- Author:
Hao WU
1
;
Wen-Tao ZHANG
;
Feng-Feng JIA
;
Ming LIU
;
Jian-Jun ZHU
Author Information
1. 山西医科大学基础医学院医学细胞生物与遗传学教研室,太原 030001
- Keywords:
laryngeal squamous cell carcinoma(LSCC);
PART1;
The Cancer Genome Atlas(TCGA)database;
long non-coding RNAs(lncRNA);
bioinformatics analysis
- From:
Chinese Journal of Biochemistry and Molecular Biology
2024;40(7):976-986
- CountryChina
- Language:Chinese
-
Abstract:
Long non-coding RNA(lncRNA)PART1,a competing endogenous RNA(ceRNA),plays a crucial role in the occurrence and development of various tumors.However,research on PART1 in laryngeal squamous cell carcinoma(LSCC)remains scarce.Based on preliminary lncRNA sequencing data,we found that PART1 was sig-nificantly downregulated in LSCC tissues.Further analysis of sequencing and clinical data from public databases such as TCGA revealed 146 differentially expressed lncRNAs(95 upregulated and 51 downregulated)and 2 424 differentially expressed mRNAs when comparing LSCC tumor and adjacent tissues.The results showed that PART1 was generally downregulated in LSCC(P＜0.0001),and patients with high PART1 expression had significantly better prognosis(P＜0.05).We used bioinformatics methods to construct the ceRNA regulatory network of PART1 in LSCC and identified the miRNAs and mRNAs interacting with it.Under laboratory conditions,the im-portance of PART1 in LSCC cells was validated in vitro.Overexpression vectors significantly increased the expres-sion of PART1 in LSCC cells(P＜0.001).Experiments including 5-ethynyl-2'-deoxyuridine staining,apoptosis analysis,scratch healing assay,Transwell assay,and phalloidin staining showed that overexpression of PART1 sig-nificantly affected the proliferation,apoptosis,migration,and invasion of LSCC cells in vitro(P＜0.001).There-fore,PART1 may suppress the occurrence and development of LSCC.This study provides a theoretical basis for e-lucidating the role of PART1 in LSCC.
