Novel Immune-related Proteins Identified from Mytilus coruscus by Hemocytes Full-length Transcriptome and Serum Differential Proteome
10.13865/j.cnki.cjbmb.2024.05.1058
- VernacularTitle:基于血细胞全长转录物组和血清差异蛋白质组的厚壳贻贝新型免疫相关蛋白质鉴定
- Author:
Wen-Hui XIAO
1
;
Hao-Dong WANG
;
Zong-Xin YANG
;
Fang SONG
;
Yue WANG
;
Jian-Yu HE
;
Xiao-Lin ZHANG
;
Xiao-Jun YAN
;
Zhi LIAO
Author Information
1. 浙江海洋大学海洋科学与技术学院,海洋生物资源与分子工程研究室,浙江舟山 316022
- Keywords:
Mytilus coruscus;
hemolymph;
full-length transcriptomics;
differential proteomics;
immuni-zation
- From:
Chinese Journal of Biochemistry and Molecular Biology
2024;40(7):947-963
- CountryChina
- Language:Chinese
-
Abstract:
Mytilus is one of bivalves with great economic and ecological values.The innate immune de-fense of Mytilus shows great significance in the study of marine biological immunology.Hemolymph is the main immune tissue for Mytilus.The Nanopore full-length transcriptome of Mytilus coruscus hemocytes,and the serum differential proteomics based on SDS-PAGE analysis were performed to identify key pro-teins involving in the immune response of Mytilus hemolymph in response of different bacteria and fungi stresses.A total of 44 proteins were identified in the serum induced by different microorganisms.Among them,26 proteins showed significant differential expression level in response to different microbial stres-ses,and their functions were involved in protein folding protection,cell autophagy and apoptosis regula-tion,reactive oxygen species production,energy metabolism regulation,cell detoxification,and immune regulation.The changes in expression levels of these proteins varied in response to different bacterial and fungal stresses,suggesting that Mytilus has different immune response strategies to different bacterial and fungal stresses.The results provide a new scientific basis for understanding the differential immune mech-anism of Mytilus innate immune system in response to different types of microbial invasion,as well as the screening of specific biomarker proteins for microbial infection,and provide ideas for the healthy develop-ment and disease prevention of shellfish aquaculture.
