Isolation and identification of genotype A Chlamydia psittaci strain from duck
10.3969/j.issn.1002-2694.2024.00.122
- VernacularTitle:鸭源A型鹦鹉热衣原体的分离鉴定
- Author:
Zhao-Cai LI
1
;
Ping LIU
;
Yun-Hui LI
;
Huai-Yu ZHANG
;
Qian XIAO
;
Chun-Guo LIU
;
Ji-Zhang ZHOU
Author Information
1. 中国农业科学院兰州兽医研究所,动物疫病防控全国重点实验室,兰州 730046
- Keywords:
Chlamydia psittaci;
cell culture;
genoty-ping;
duck
- From:
Chinese Journal of Zoonoses
2024;40(9):823-828
- CountryChina
- Language:Chinese
-
Abstract:
Chlamydia psittaci is a worldwide distributed zoonotic pathogen that infects a variety of birds.In order to char-acterization of the duck originated C.psittaci strains,lung samples were collected from suspected infection ducks and was de-tected by real-time PCR.The positive samples were homogenized in phosphate buffered saline with kanamycin and streptomy-cin,and then inoculated onto L929 cells monolayer.After several sets of passages,chlamydial inclusions of the isolate in cul-tured cells were observed after Giemsa staining or by electron microscope.For species identification,16S rRNA,16-23S IGS gene fragments were sequenced and analyzed.Genotyping of the isolate was performed by comparative analysis of the obtained ompA gene sequence with that of different genotype of C.psittaci strains.A C.psittaci strain was successfully isolated from the lung sample of duck by cell culture and was identified as genotype A.This study expanded our understanding of the host range of genotype A C.psittaci strain,and provided basis for further research on the pathogenicity,transmission,and public health risk of this pathogen.
