Multiple cross displacement amplification coupled with a gold nanoparticle-based lateral flow biosensor for detection of Listeria ivanovii
10.3969/j.issn.1002-2694.2024.00.098
- VernacularTitle:多交叉置换扩增结合纳米生物传感器检测伊氏李斯特菌的方法建立
- Author:
Hui LI
1
;
Yi WANG
;
Yan WANG
;
Chang-Yun YE
Author Information
1. 山西医科大学汾阳学院病原生物学与检验教研室,汾阳 032200
- Keywords:
Listeria ivanovii;
multiple cross displace-ment amplification;
lateral flow biosensor;
smcl gene;
limit of detection
- From:
Chinese Journal of Zoonoses
2024;40(7):605-612
- CountryChina
- Language:Chinese
-
Abstract:
A method using multiple cross displacement amplification combined with a lateral flow biosensor to detect Liste-ria ivanovii was developed and evaluated in this study.The L.ivanovii-MCDA-LFB assay used a set of ten primers spanning ten distinct regions of smcl,and was performed at a constant temperature of 64 ℃ to evaluate its specificity,sensitivity and feasibility.The specificity of MCDA-LFB assay was 100％in L.ivanovii and non-L.ivanovii strains.The limit of detection of the MCDA-LFB assay in pure culture and in L.ivanovii artificial contaminated feces was 10 fg and 6.8× 102 CFU/g,respec-tively.The detection results of the L.ivanovii-MCDA-LFB assay were identical to those of traditional culture-based methods(ISO 11290-1)in 195 wild rat intestinal feces samples.Detection could be achieved by boiling the template with the second en-richment solution.Thus,our rapid L.ivanovii-MCDA-LFB method with favorable sensitivity and specificity may provide a useful tool for detecting L.ivanovii in food surveillance,and medical and laboratory research.
