Virome characteristics and monkeypox virus screening of artificially domesticated primates in the Guangdong region
10.3969/j.issn.1002-2694.2024.00.066
- VernacularTitle:广东地区人工驯养灵长动物的病毒组特征及猴痘病毒筛查
- Author:
Na LI
1
;
Zhao-Wen REN
;
Pian ZHANG
;
Zi-Guo YUAN
;
Xiao-Fan CHEN
;
Ming LIAO
;
Xiao-Hu WANG
Author Information
1. 广东省农业科学院动物卫生研究所;广东省畜禽疫病防治研究重点实验室;农业农村部兽用药物与诊断技术广东科学观测实验站,广州 510640;华南农业大学兽医学院,广州 510640
- Keywords:
primates;
viral metagenome;
monkeypox virus;
zoonotic diseases;
fluorescence quantitative PCR
- From:
Chinese Journal of Zoonoses
2024;40(5):391-400
- CountryChina
- Language:Chinese
-
Abstract:
To clarify the structural characteristics of virus communities carried by primates in the Guangdong region,and evaluate the risk of the important zoonotic virus monkeypox virus(MPXV)being introduced into China through artificially do-mesticated primates,this study conducted metagenomic research on artificially domesticated primates and performed screening for MPXV.Primate samples were collected from 20 wildlife rescue centers or zoos in 14 prefecture level cities in Guangdong Province,and the structural characteristics of virus communities carried by artificially domesticated primates were identified through Illumina sequencing.Fluorescence quantitative PCR detection of MPXV excluded the risk of MPXV being introduced through artificially domesticated primates in Guangdong Prov-ince.A total of 489 oral and pharyngeal swabs and feces from primates were collected.High-throughput sequencing indicated that the viral group structure in the feces of artificially domesti-cated primates in the Guangdong region is complex and shows regional differences.Members of Alphaflexiviridae and Vir-gaviridae,followed by members of Parvoviridae and Genomo-viridae,had the highest abundance.Subsequently,fluorescence quantitative PCR results showed that all primates from wildlife rescue centers or zoos in Guangdong Province were MPXV neg-ative.This study provides the first description of the complex viral structure characteristics of artificially domesticated primates in the Guangdong region,and elucidates the differences in vi-ral communities among artificially domesticated primates in different regions.Our findings suggested that the risk of zoonotic diseases caused by artificially domesticated primates in Guangdong Province is extremely low,and the risk of MPXV being in-troduced into China through artificially domesticated primates in Guangdong Province is zero.
