Clinical value of serum miRNA-369 and miRNA-597 in predicting postoperative recurrence and metastasis of laparoscopic radical resection for colon cancer
- VernacularTitle:血清微RNA-369和微RNA-597预测腹腔镜结肠癌根治术后复发转移的临床价值
- Author:
Zhijie XU
1
Author Information
- Keywords: micro RNA-369(miRNA-369); micro RNA-597(miRNA-597); colon cancer; laparoscopic radical surgery; recurrence and metastasis
- From: China Journal of Endoscopy 2024;30(11):74-81
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the predictive value of serum microRNA-369(miRNA-369)and microRNA-597(miRNA-597)for recurrent and metastasis after laparoscopic radical resection for colon cancer.Methods 128 colon cancer patients with laparoscopic radical resection from September 2020 to September 2022 were regarded as the colon cancer group.Based on the recurrence rate of the patients after laparoscopic surgery for 1 year,they were separated into two groups:recurrence and metastasis group(n=30)and non recurrence and metastasis group(n=98),eighty-six patients with benign colonic lesions who visited our hospital during the same period were selected as control group.Real time fluorescent quantitative polymerase chain reaction(RT-qPCR)method was applied to detect the expression levels of serum miRNA-369 and miRNA-597 in each group.Multivariate Logistic regression analysis of factors influencing recurrence and metastasis after laparoscopic radical resection for colon cancer.Receiver operator characteristic curve(ROC curve)was plotted to assess the predictive value of serum miRNA-369 and miRNA-597 levels for recurrence and metastasis after laparoscopic radical resection for colon cancer.Results The expression levels of serum miRNA-369 and miRNA-597 in the colon cancer group were obviously lower than those in the control group(P<0.05).The degree of tumour differentiation,TNM stage,lymph node metastasis,expression levels of reactive oxygen species(ROS),interleukin-6(IL-6),interleukin-22(IL-22),miRNA-369 and miRNA-597 between the recurrence and metastasis group and non recurrence and metastasis group,with significant differences(P<0.05).Multivariate Logistic regression analysis showed that:TNM staging III-IV,low differentiation,lymph node metastasis,elevated levels of ROS≥1 120.88 u/mL,IL-6≥37.97 ng/mL,IL-22≥35.93 pg/mL,miRNA-369<0.48 and miRNA-597<0.66 were risk factors for postoperative recurrence and metastasis in colon cancer patients of laparoscopic radical resection(P<0.05).The area under the curve(AUC)of serum miRNA-369 and miRNA-597 alone and in combination for predicting postoperative recurrence and metastasis of laparoscopic radical resection for colon cancer was 0.897,0.843,and 0.950,respectively,the combined predictive effect was better than that of single prediction(Zcombination with miRNA-369=2.04,Pcombination with miRNA-369=0.041,Zcombination with miRNA-597=2.96,Pcombination with miRNA-597=0.003).Conclusion Serum miRNA-369 and miRNA-597 are both significantly reduced in expression in colon cancer patients,and they have high predictive value for recurrence and metastasis after laparoscopic radical resection for colon cancer.
