Influences of echinacoside on myocardial cell apoptosis and inflammatory injury in rats with coronary heart disease by regulating mTOR/STAT3 signaling pathway
10.3969/j.issn.1000-484X.2024.12.019
- VernacularTitle:松果菊苷调节mTOR/STAT3信号通路对冠心病大鼠心肌细胞凋亡和炎症损伤的影响
- Author:
Qiang SU
1
;
Jiahe XIE
;
He HUANG
;
Shan ZENG
;
Yiming ZHONG
Author Information
1. 赣南医学院第一附属医院心血管内科,赣南医学院心脑血管疾病防治教育部重点实验室,赣州 341000
- Keywords:
Echinacoside;
mTOR/STAT3 pathway;
Coronary heart disease;
Inflammatory;
Cell apoptosis
- From:
Chinese Journal of Immunology
2024;40(12):2574-2579
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate influences of echinacoside(ECH)on myocardial cell apoptosis,inflammatory injury and mammalian target of rapamycin(mTOR)/signal transducer and activator of transcription 3(STAT3)signaling pathway in rats with coronary heart disease(CHD).Methods:A total of 12 SD rats were taken as control group(NC group),48 CHD rat models were successfully constructed,and randomly divided into model(Model)group,ECH group(50 mg/kg),mTOR activator MHY1485 group(10 mg/kg),ECH+MHY1485 group(50 mg/kg ECH+10 mg/kg MHY1485).After 4 weeks,electrocardiogram was recorded through biological function experimental system.Kits were used to detect myocardial C-reactive protein(CRP)and IL-6 levels.Myocardial pathology and apoptosis were measured by HE and TUNEL staining.qRT-PCR was used to detect myocardial Bcl-2,Bax mRNA expressions.Western blot was used to detect myocardial mTOR/STAT3 pathway protein expression.Results:Compared with NC group,Model group rats had obvious necrosis,edema and inflammatory cell infiltration,ST segment deviation,IL-6,CRP levels,cardiomyocyte apoptosis rate,Bax mRNA expression,mTOR and p-STAT3/STAT3 protein levels were significantly increased(P<0.05),Bcl-2 mRNA expression was significantly decreased(P<0.05).Compared with Model group,myocardial injury in ECH group was alleviated,ST segment shift,IL-6,CRP levels,cardiomyocyte apoptosis rate,Bax mRNA expression,mTOR and p-STAT3/STAT3 protein levels were were significantly decreased(P<0.05),Bcl-2 mRNA expression was significantly increased(P<0.05).The above indexes of MHY1485 group rats showed opposite trend.MHY1485 eliminates beneficial effects of ECH on CHD rats.Conclusion:ECH may reduce myocardial inflammatory injury and apoptosis in CHD rats by down-regulating mTOR/STAT3 pathway.
